RAPD-PCR experiments confirm the distinction between three morphologically similar species of Nerilla (Polychaeta : Nerillidae)

Autor(en): Schmidt, H
Westheide, W
Stichwörter: AMPLIFIED POLYMORPHIC DNA; BOOTSTRAP; CONFIDENCE; cosmopolitan species; IDENTIFICATION; MARINE ORGANISMS; meiofauna; molecular taxonomy; Nerilla; polychaeta; PRIMERS; SYSTEMATICS; Zoology
Erscheinungsdatum: 1998
Herausgeber: ELSEVIER GMBH
Volumen: 236
Ausgabe: 4
Startseite: 277
Seitenende: 285
By means of the random amplified polymorphic DNA (RAPD)-PCR technique employing 12 different random oligonucleotide primers, it has been shown that three species of the meiofaunal polychaete taxon Nerilla that had been established on the basis of slight differences in morphological characters, N. antennata Schmidt, 1848 (North Sea, Helgoland), N. mediterranea Schlieper, 1925 (Atlantic coast of Spain, Mediterranean coast of Italy) and N. inopinata Gray, 1968 (Pacific, Washington State in the USA, Chinese coast in the Strait of Taiwan), are also clearly distinct genetically. An amphipacific distribution of the last species is made most likely by UPGMA and neighbour-joining phenograms although the American and Chinese animals show slight morphological differences.
ISSN: 00445231

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