CALIBRATION AND TIME RESOLUTION OF LUMENAL PH-TRANSIENTS IN CHROMATOPHORES OF RHODOBACTER-CAPSULATUS FOLLOWING A SINGLE TURNOVER FLASH OF LIGHT - PROTON RELEASE BY THE CYTOCHROME BC(1)-COMPLEX IS STRONGLY ELECTROGENIC
Autor(en): | MULKIDJANIAN, AY JUNGE, W |
Stichwörter: | ACCEPTOR; Biochemistry & Molecular Biology; Biophysics; CAPACITY; Cell Biology; CYTOCHROME BC(1)-COMPLEX; EVENTS; MAGNITUDE; MEMBRANE POTENTIAL; PROTON GRADIENT; PROTON/ELECTRON TRANSFER; RHODOBACTER CAPSULATUS; SPHAEROIDES CHROMATOPHORES; UBIQUINOL | Erscheinungsdatum: | 1994 | Herausgeber: | ELSEVIER SCIENCE BV | Journal: | FEBS LETTERS | Volumen: | 353 | Ausgabe: | 2 | Startseite: | 189 | Seitenende: | 193 | Zusammenfassung: | The flash-induced proton release into the lumen of chromatophores from Rhodobacter capsulatus was studied with Neutral red as pH-indicator. Calibration of the acidification jump after a single flash yielded a much larger figure, at least 0.8 units, than previously thought. A slow kinetic phase of proton release (85-90% of total) was sensitive to inhibitors of the cytochrome bc(1)-complex. Its half-rise time, about 10 ms, was the same as the rise time of the electrogenic reaction in the cytochrome bc(1)-complex that was recorded by electrochromism of carotenoids. The oxidoreduction of the two b-hemes was significantly faster (t(1/2) congruent to 3 ms). Thus the major electrogenic event in the cytochome bc(1)-complex is proton and not electron transfer. |
ISSN: | 00145793 | DOI: | 10.1016/0014-5793(94)01031-5 |
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