RNA Protein Granules Modulate tau Isoform Expression and Induce Neuronal Sprouting

DC FieldValueLanguage
dc.contributor.authorMoschner, Katharina
dc.contributor.authorSuendermann, Frederik
dc.contributor.authorMeyer, Heiko
dc.contributor.authorda Graca, Abel Pereira
dc.contributor.authorAppel, Neele
dc.contributor.authorPaululat, Achim
dc.contributor.authorBakota, Lidia
dc.contributor.authorBrandt, Roland
dc.date.accessioned2021-12-23T16:03:32Z-
dc.date.available2021-12-23T16:03:32Z-
dc.date.issued2014
dc.identifier.issn00219258
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/6049-
dc.description.abstractThe neuronal microtubule-associated protein Tau is expressed in different variants, and changes in Tau isoform composition occur during development and disease. Here, we investigate a potential role of the multivalent tau mRNA-binding proteins G3BP1 and IMP1 in regulating neuronal tau expression. We demonstrate that G3BP1 and IMP1 expression induces the formation of structures, which qualify as neuronal ribonucleoprotein (RNP) granules and concentrate multivalent proteins and mRNA. We show that RNP granule formation leads to a >30-fold increase in the ratio of high molecular weight to low molecular weight tau mRNA and an similar to 12-fold increase in high molecular weight to low molecular weight Tau protein. We report that RNP granule formation is associated with increased neurite formation and enhanced process growth. G3BP1 deletion constructs that do not induce granule formation are also deficient in inducing neuronal sprouting or changing the expression pattern of tau. The data indicate that granule formation driven by multivalent proteins modulates tau isoform expression and suggest a morphoregulatory function of RNP granules during health and disease.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 944]; Lichtenberg fellowship; This work was supported by Deutsche Forschungsgemeinschaft Grant SFB 944, Project P1 (to R. B.), and a Lichtenberg fellowship (to F. S.).
dc.language.isoen
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.relation.ispartofJOURNAL OF BIOLOGICAL CHEMISTRY
dc.subjectASSOCIATION
dc.subjectBiochemistry & Molecular Biology
dc.subjectCENTRAL-NERVOUS-SYSTEM
dc.subjectCORTICAL-NEURONS
dc.subjectGENE
dc.subjectKH DOMAINS
dc.subjectLOCALIZATION
dc.subjectMEMBRANE
dc.subjectMESSENGER-RNA
dc.subjectREVEALS
dc.subjectSTRESS GRANULES
dc.titleRNA Protein Granules Modulate tau Isoform Expression and Induce Neuronal Sprouting
dc.typejournal article
dc.identifier.doi10.1074/jbc.M113.541425
dc.identifier.isiISI:000338016200019
dc.description.volume289
dc.description.issue24
dc.description.startpage16814
dc.description.endpage16825
dc.contributor.orcid0000-0003-0101-1257
dc.contributor.orcid0000-0002-8845-6859
dc.contributor.orcid0000-0002-8777-5006
dc.contributor.orcid0000-0002-3304-4523
dc.identifier.eissn1083351X
dc.publisher.place11200 ROCKVILLE PIKE, SUITE 302, ROCKVILLE, MD, UNITED STATES
dcterms.isPartOf.abbreviationJ. Biol. Chem.
dcterms.oaStatushybrid, Green Published
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-8845-6859-
crisitem.author.orcid0000-0003-0101-1257-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPaAc947-
crisitem.author.netidBrRo587-
Show simple item record

Page view(s)

5
Last Week
0
Last month
0
checked on Apr 19, 2024

Google ScholarTM

Check

Altmetric