The mtl genes and the mannitol-l-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026

DC FieldValueLanguage
dc.contributor.authorOtte, S
dc.contributor.authorLengeler, JW
dc.date.accessioned2021-12-23T16:03:38Z-
dc.date.available2021-12-23T16:03:38Z-
dc.date.issued2001
dc.identifier.issn03781097
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/6103-
dc.description.abstractThe mtl operon of Klebsiella pneumoniae KAY2076 (formerly Aerobacter aerogenes 1033-5P14) was shown to contain as the promoter-proximal gene mtlA, encoding a D-mannitol-specific enzyme II transporter (IICBA(Mtl)). This gene is followed by mtlD, coding for a mannitol-1-phosphate dehydrogenase (MtlD, 382 amino acid residues), and mtlR (MtlR, 195 amino acid residues) coding for a putative repressor, gene mtlR overlaps the termination codon of mtlD. The DNA and protein sequences are highly similar to the corresponding genes (81%, identical bp) and proteins (79-85% identical amino acids) of Escherichia coli K-12. A truncated form of MtlD lacking the 162 C-terminal amino acid residues still shows 10% dehydrogenase activity which may explain the controversy in the literature concerning the properties of mannitol-phosphate and other medium-length dehydrogenases. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofFEMS MICROBIOLOGY LETTERS
dc.subjectBACTERIOPHAGE-T7 RNA-POLYMERASE
dc.subjectCHAIN ALCOHOL DEHYDROGENASES
dc.subjectCLONING
dc.subjectDEPENDENT PHOSPHOTRANSFERASE SYSTEM
dc.subjectENZYMES
dc.subjectESCHERICHIA-COLI K-12
dc.subjectEXPRESSION
dc.subjectKlebsiella pneumoniae
dc.subjectmannitol
dc.subjectMANNITOL-1-PHOSPHATE DEHYDROGENASE
dc.subjectmannitol-l-phosphate dehyrogenase
dc.subjectMicrobiology
dc.subjectmtl gene
dc.subjectNUCLEOTIDE-SEQUENCE
dc.subjectTRANSPORT
dc.titleThe mtl genes and the mannitol-l-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026
dc.typejournal article
dc.identifier.doi10.1111/j.1574-6968.2001.tb09473.x
dc.identifier.isiISI:000166705500018
dc.description.volume194
dc.description.issue2
dc.description.startpage221
dc.description.endpage227
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationFEMS Microbiol. Lett.
dcterms.oaStatusBronze
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