Electrogenicity of electron and proton transfer at the oxidizing side of photosystem II

DC FieldValueLanguage
dc.contributor.authorHaumann, M
dc.contributor.authorMulkidjanian, A
dc.contributor.authorJunge, W
dc.date.accessioned2021-12-23T16:03:40Z-
dc.date.available2021-12-23T16:03:40Z-
dc.date.issued1997
dc.identifier.issn00062960
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/6118-
dc.description.abstractThe electrogenicity of electron and proton transfer at the oxidizing side of PSII was monitored by transmembrane electrochromism of carotenoids in thylakoids and, independently, by electrometry in oxygen-evolving photosystem II core particles. It yielded dielectrically weighted distances between cofactors, They were related to the one between Y-Z(ox) and Q(A)(-) (=100%). The electron transfer from Y-Z to P-680(+) ranged over a relative distance of 15%, while the one from Mn-4 to Y-Z(ox) ranged over less than 3.5%. The latter result placed Mn-4 and Y-Z at about the same weighted depth in the membrane. The oxidation of cofactor X by Y-Z(ox) during S-2 --> S-3 ranged over 10%. We tentatively attributed 7% to proton transfer into the lumen and 3% to electron transfer, in line with our notion that one proton is liberated from X-ox itself. This placed X at the same depth in the membrane as Mn. Proton release upon the final oxidation of water during the oxygen-evolving step S-4 --> S-0 revealed relative electrogenic components of 5.5% in care particles and between 10.5% (pH 7.4) and 2% (pH 6.2) in thylakoids. The former likely reflected proton transfer from bound water into the lumen and the latter to intraprotein bases that were created in the foregoing transitions, A tentative scheme for the arrangement of cofactors al the oxidizing side of photosystem II is presented.
dc.language.isoen
dc.publisherAMER CHEMICAL SOC
dc.relation.ispartofBIOCHEMISTRY
dc.subjectBiochemistry & Molecular Biology
dc.subjectHISTIDINE OXIDATION
dc.subjectMANGANESE
dc.subjectOXYGEN-EVOLVING COMPLEX
dc.subjectPARAMAGNETIC-RESONANCE
dc.subjectPARTICLES
dc.subjectPHOTOSYNTHETIC WATER OXIDATION
dc.subjectREDOX-ACTIVE TYROSINE
dc.subjectREDUCTION KINETICS
dc.subjectRELEASE
dc.subjectTHYLAKOID MEMBRANES
dc.titleElectrogenicity of electron and proton transfer at the oxidizing side of photosystem II
dc.typejournal article
dc.identifier.doi10.1021/bi963114p
dc.identifier.isiISI:A1997XP63100004
dc.description.volume36
dc.description.issue31
dc.description.startpage9304
dc.description.endpage9315
dc.contributor.orcid0000-0001-5844-3064
dc.contributor.researcheridJ-8086-2013
dc.contributor.researcheridAAH-3608-2021
dc.contributor.researcheridA-7087-2013
dc.publisher.place1155 16TH ST, NW, WASHINGTON, DC 20036 USA
dcterms.isPartOf.abbreviationBiochemistry
crisitem.author.deptUniversität Osnabrück-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-5844-3064-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidMuAr001-
crisitem.author.netidJuWo587-
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