CHARACTERIZATION OF GLUCOSE-6-PHOSPHATE INCORPORATION INTO STARCH BY ISOLATED INTACT CAULIFLOWER-BUD PLASTIDS
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | NEUHAUS, HE | |
dc.contributor.author | HENRICHS, G | |
dc.contributor.author | SCHEIBE, R | |
dc.date.accessioned | 2021-12-23T16:03:42Z | - |
dc.date.available | 2021-12-23T16:03:42Z | - |
dc.date.issued | 1993 | |
dc.identifier.issn | 00320889 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/6142 | - |
dc.description.abstract | Intact plastids from cauliflower (Brassica oleracea var Prince de Bretagne) buds were isolated according to the method described by Journet and Douce (E.P. Journet and R. Douce [1985] Plant Physiol 79: 458-467). Incubation of these plastids with various C-14-labeled compounds revealed that glucose-6-phosphate can act as a precursor f or starch synthesis. However, significant rates (incorporation of 120 nmol glucose mg-1 protein h-1) could only be observed when both 3-phosphoglyceric acid and ATP were present as well. Starch synthesis in isolated plastids was strongly dependent upon the intactness of the organelle. The presence of a high-affinity ATP/ADP translocator with a K(m) for ATP of 12 mum was demonstrated by uptake experiments with [C-14]ATP. ADP inhibited both ATP uptake and effector-stimulated starch synthesis. Effector-stimulated glucose-6-phosphate-dependent starch synthesis was not significantly influenced by fructose-6-phosphate or 2-deoxyglucose-6-phosphate but was strongly inhibited by triose phosphate and inorganic phosphate. Starch synthesis was also inhibited by 4,4'-diisothio-cyanostilbene-2,2'-disulfonate, which is known to be a potent inhibitor of the chloroplast phosphate translocator. The data presented here support the view that starch biosynthesis in heterotrophic tissues is powered by increasing levels of cytosolic 3-phosphoglyceric acid and ATP when glucose-6-phosphate is available. | |
dc.language.iso | en | |
dc.publisher | OXFORD UNIV PRESS INC | |
dc.relation.ispartof | PLANT PHYSIOLOGY | |
dc.subject | ADENYLATE TRANSLOCATOR | |
dc.subject | ADP-GLUCOSE | |
dc.subject | AMYLOPLASTS | |
dc.subject | BIOSYNTHESIS | |
dc.subject | CHENOPODIUM-RUBRUM | |
dc.subject | ENZYMIC CAPACITIES | |
dc.subject | INNER MEMBRANE | |
dc.subject | INORGANIC-PHOSPHATE | |
dc.subject | Plant Sciences | |
dc.subject | SOLANUM-TUBEROSUM | |
dc.subject | TRIOSE PHOSPHATES | |
dc.title | CHARACTERIZATION OF GLUCOSE-6-PHOSPHATE INCORPORATION INTO STARCH BY ISOLATED INTACT CAULIFLOWER-BUD PLASTIDS | |
dc.type | journal article | |
dc.identifier.doi | 10.1104/pp.101.2.573 | |
dc.identifier.isi | ISI:A1993KM09400031 | |
dc.description.volume | 101 | |
dc.description.issue | 2 | |
dc.description.startpage | 573 | |
dc.description.endpage | 578 | |
dc.identifier.eissn | 15322548 | |
dc.publisher.place | JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA | |
dcterms.isPartOf.abbreviation | Plant Physiol. | |
dcterms.oaStatus | Bronze, Green Published | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-6140-6181 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | ScRe288 | - |
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