SULFATE METABOLISM IN ENTAMOEBA-HISTOLYTICA

Autor(en): BAKKERGRUNWALD, T
GEILHORN, B
Stichwörter: Biochemistry & Molecular Biology; CHOLESTERYL SULFATE; ENTAMOEBA-HISTOLYTICA; Parasitology; SULFATE ESTER; SULFATE TRANSPORT
Erscheinungsdatum: 1992
Herausgeber: ELSEVIER SCIENCE BV
Journal: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volumen: 53
Ausgabe: 1-2
Startseite: 71
Seitenende: 78
Zusammenfassung: 
Sulfate fluxes and sulfate metabolites in Entamoeba histolytica were characterized employing [S-35]sulfate as a marker. Sulfate was taken up both across the plasma membrane and by pinocytosis; in growth medium (sulfate concentration, 1.1 mM) total uptake was 1.5-mu-mol h-1 (5 x 10(7) cells)-1. The fate of sulfate within the cells was investigated by thin-layer chromatography. Major metabolites (together > 3-mu-mol (5 x 10(7) cells)-1) were monoethyl sulfate and 3-cholesteryl sulfate; both these products were released into the growth medium. As minor components we identified the activated sulfate derivatives, adenosine-5'-phosphosulfate and 3'-phosphoadenosine-5'-phosphosulfate. In addition, up to 10% of the sulfate taken up was incorporated into high-molecular weight material (possibly proteoglycans). We propose that sulfurylation of cholesterol may play a role in controlling membrane sterol content.
ISSN: 01666851
DOI: 10.1016/0166-6851(92)90008-8

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