Probing protein palmitoylation at the yeast vacuole

Autor(en): Meiringer, Christoph T. A.
Ungermann, Christian 
Stichwörter: ALPHA-SNAP; Biochemical Research Methods; Biochemistry & Molecular Biology; EARLY-STAGE; IN-VITRO REACTIONS; MEMBRANE-FUSION; palmitoylation; R-SNARE; SACCHAROMYCES-CEREVISIAE; SNARE COMPLEX; TRANSPORT PATHWAYS; V-SNARES; VAC8P; vacuole
Erscheinungsdatum: 2006
Herausgeber: ACADEMIC PRESS INC ELSEVIER SCIENCE
Journal: METHODS
Volumen: 40
Ausgabe: 2
Startseite: 171
Seitenende: 176
Zusammenfassung: 
A protein's function depends on its localization to the right cellular compartment. A number of proteins require lipidation to associate with membranes. Protein palmitoylation is a reversible lipid modification and has been shown to mediate both membrane localization and control protein function. At the yeast vacuole, several palmitoylated proteins have been identified that are required for vacuole biogenesis, including the fusion factor Vac8, the SNARE Ykt6 and the casein kinase Yck3. Moreover, both the DHHC-CRD acyltransferase Pfa3 and Ykt6 are involved in palmitoylation at the vacuole Here, we present and discuss methods to probe for protein palmitoylation at vacuoles. (c) 2006 Elsevier Inc. All rights reserved.
ISSN: 10462023
DOI: 10.1016/j.ymeth.2006.06.020

Show full item record

Google ScholarTM

Check

Altmetric