Profiling early osmostress-dependent gene expression in Escherichia coli using DNA microarrays

Autor(en): Weber, A
Jung, K
Stichwörter: BINDING PROTEIN; IDENTIFICATION; INTEGRATION HOST FACTOR; K-12; Microbiology; OSMOREGULATION; OSMOTIC-STRESS; PROLINE TRANSPORT; RPOS; STATIONARY-PHASE; TURGOR PRESSURE
Erscheinungsdatum: 2002
Herausgeber: AMER SOC MICROBIOLOGY
Journal: JOURNAL OF BACTERIOLOGY
Volumen: 184
Ausgabe: 19
Startseite: 5502
Seitenende: 5507
Zusammenfassung: 
DNA macroarray technology was used to monitor early transcriptional alterations of Escherichia coli in response to an osmotic upshift imposed by the addition of 0.4 M NaCl. Altered mRNA levels of 152 genes were detected; 45 genes showed increased expression while the expression of the remaining 107 genes was reduced. Northern blot analysis of several selected genes differing in their relative expression values confirmed the results obtained by the array technology.
ISSN: 00219193
DOI: 10.1128/JB.184.19.5502-5507.2002

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