7-DEAZA-2'-DEOXYADENOSINE AND 3-DEAZA-2'-DEOXYADENOSINE REPLACING DA WITHIN D(A6)-TRACTS - DIFFERENTIAL BENDING AT 3'-JUNCTION AND 5'-JUNCTION OF D(A6)BULLET-D(T6) AND B-DNA

Autor(en): SEELA, F
GREIN, T
Stichwörter: 2'-DEOXYTUBERCIDIN; BASE; Biochemistry & Molecular Biology; CHROMATIN DNA; CURVED DNA; GLYCOSYLATION; HYDRATION; MINOR-GROOVE; OLIGONUCLEOTIDES; SEQUENCE; THYMINE TRACTS
Erscheinungsdatum: 1992
Herausgeber: OXFORD UNIV PRESS
Journal: NUCLEIC ACIDS RESEARCH
Volumen: 20
Ausgabe: 13
Startseite: U3549-U3558
Zusammenfassung: 
7-Deaza-2'-deoxyadenosine (1, C7A(d)) and 3-deaza-2'-deoxyadenosine (2, C3A(d)) have been incorporated into d(AAAAAA) tracts replacing dA at various positions within oligonucleotides. For this purpose suitably protected phosphonates have been prepared and oligonucleotides were synthsized on solid-phase. The oligomers were hybridized with their cognate strands. The duplexes were phosphorylated at OH-5' by polynucleotide kinase and self-ligated to multimers employing T4 DNA ligase. Oligomerized DNA-fragments were analyzed by polyacrylamide gel electrophoresis and the bending was determined from anomalies of electrophoretic mobility. Replacement of dA by C3A(d) decreased the bending more than replacement by C7A(d). Reduction of bending was much stronger when the modified nucleosides replaced one or several dA residues at the 3'-site of an d(AAAAAA)-tract whereas replacement at the 5'-site showed no significant influence [1, 2].
ISSN: 03051048
DOI: 10.1093/nar/20.13.3549

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