Proteomes of Host Cell Membranes Modified by Intracellular Activities of Salmonella enterica

DC FieldValueLanguage
dc.contributor.authorVorwerk, Stephanie
dc.contributor.authorKrieger, Viktoria
dc.contributor.authorDeiwick, Joerg
dc.contributor.authorHensel, Michael
dc.contributor.authorHansmeier, Nicole
dc.date.accessioned2021-12-23T16:04:22Z-
dc.date.available2021-12-23T16:04:22Z-
dc.date.issued2015
dc.identifier.issn15359476
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/6393-
dc.description.abstractIntracellular pathogens need to establish a growth-stimulating host niche for survival and replication. A unique feature of the gastrointestinal pathogen Salmonella enterica serovar Typhimurium is the creation of extensive membrane networks within its host. An understanding of the origin and function of these membranes is crucial for the development of new treatment strategies. However, the characterization of this compartment is very challenging, and only fragmentary knowledge of its composition and biogenesis exists. Here, we describe a new proteome-based approach to enrich and characterize Salmonella-modified membranes. Using a Salmonella mutant strain that does not form this unique membrane network as a reference, we identified a high-confidence set of host proteins associated with Salmonella-modified membranes. This comprehensive analysis allowed us to reconstruct the interactions of Salmonella with host membranes. For example, we noted that Salmonella redirects endoplasmic reticulum (ER) membrane trafficking to its intracellular niche, a finding that has not been described for Salmonella previously. Our system-wide approach therefore has the potential to rapidly close gaps in our knowledge of the infection process of intracellular pathogens and demonstrates a hitherto unrecognized complexity in the formation of Salmonella host niches.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [HE1964/18-1, SPP1580]; Niedersachsisches Ministerium fur Wissenschaft und Kultur; This work was supported by Grant No. HE1964/18-1 within Priority Program SPP1580 of the Deutsche Forschungsgemeinschaft and by the Niedersachsisches Ministerium fur Wissenschaft und Kultur.
dc.language.isoen
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.relation.ispartofMOLECULAR & CELLULAR PROTEOMICS
dc.subjectBiochemical Research Methods
dc.subjectBiochemistry & Molecular Biology
dc.subjectCONTAINING VACUOLES
dc.subjectDATABASE
dc.subjectDYNAMICS
dc.subjectEFFECTOR PROTEIN
dc.subjectF-ACTIN
dc.subjectIII SECRETION SYSTEM
dc.subjectINDUCED FILAMENTS
dc.subjectINVASION
dc.subjectPATHOGENICITY ISLAND-2
dc.subjectRAB GTPASES
dc.titleProteomes of Host Cell Membranes Modified by Intracellular Activities of Salmonella enterica
dc.typejournal article
dc.identifier.doi10.1074/mcp.M114.041145
dc.identifier.isiISI:000347155500007
dc.description.volume14
dc.description.issue1
dc.description.startpage81
dc.description.endpage92
dc.contributor.orcid0000-0001-6239-8614
dc.contributor.orcid0000-0002-1304-2512
dc.identifier.eissn15359484
dc.publisher.place9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA
dcterms.isPartOf.abbreviationMol. Cell. Proteomics
dcterms.oaStatusGreen Published, hybrid
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6604-6253-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidHeMi480-
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