ATP-DRIVEN POTASSIUM-TRANSPORT IN RIGHT-SIDE-OUT MEMBRANE-VESICLES VIA THE KDP SYSTEM OF ESCHERICHIA-COLI
| Autor(en): | KOLLMANN, R ALTENDORF, K |
Stichwörter: | (ESCHERICHIA-COLI); ATP GENERATION; ATPASES; BACTERIA; Biochemistry & Molecular Biology; Biophysics; K+-TRANSPORT; KDP-ATPASE; OSMOREGULATION; PH; PHOSPHOGLYCERATE TRANSPORT; POTASSIUM TRANSPORT; RIGHT-SIDE-OUT MEMBRANE VESICLE | Erscheinungsdatum: | 1993 | Herausgeber: | ELSEVIER SCIENCE BV | Journal: | BIOCHIMICA ET BIOPHYSICA ACTA | Volumen: | 1143 | Ausgabe: | 1 | Startseite: | 62 | Seitenende: | 66 | Zusammenfassung: | The ATP-generating system described by Hugenholtz, J., Hong, J.-S. and Kaback, H.R. ((1981) Proc. Natl. Acad. Sci. USA 78, 3446-3449) has been used to synthesize ATP up to 1.8 mM in right-side-out membrane vesicles from Escherichia coli. This ATP level was sufficient to drive uptake of potassium ions via the Kdp-ATPase. In the kdp wild type strain about 110 nmoles K+/mg membrane protein were accumulated. This process was still partially sensitive to the well-known inhibitors of P-type ATPases, orthovanadate and bafilomycin B1. |
ISSN: | 00063002 | DOI: | 10.1016/0005-2728(93)90216-3 |
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geprüft am 17.05.2024
