ATP-DRIVEN POTASSIUM-TRANSPORT IN RIGHT-SIDE-OUT MEMBRANE-VESICLES VIA THE KDP SYSTEM OF ESCHERICHIA-COLI

Autor(en): KOLLMANN, R
ALTENDORF, K 
Stichwörter: (ESCHERICHIA-COLI); ATP GENERATION; ATPASES; BACTERIA; Biochemistry & Molecular Biology; Biophysics; K+-TRANSPORT; KDP-ATPASE; OSMOREGULATION; PH; PHOSPHOGLYCERATE TRANSPORT; POTASSIUM TRANSPORT; RIGHT-SIDE-OUT MEMBRANE VESICLE
Erscheinungsdatum: 1993
Herausgeber: ELSEVIER SCIENCE BV
Journal: BIOCHIMICA ET BIOPHYSICA ACTA
Volumen: 1143
Ausgabe: 1
Startseite: 62
Seitenende: 66
Zusammenfassung: 
The ATP-generating system described by Hugenholtz, J., Hong, J.-S. and Kaback, H.R. ((1981) Proc. Natl. Acad. Sci. USA 78, 3446-3449) has been used to synthesize ATP up to 1.8 mM in right-side-out membrane vesicles from Escherichia coli. This ATP level was sufficient to drive uptake of potassium ions via the Kdp-ATPase. In the kdp wild type strain about 110 nmoles K+/mg membrane protein were accumulated. This process was still partially sensitive to the well-known inhibitors of P-type ATPases, orthovanadate and bafilomycin B1.
ISSN: 00063002
DOI: 10.1016/0005-2728(93)90216-3

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