Effect of Environmental Temperatures on Proteome Composition of Salmonella enterica Serovar Typhimurium
DC Element | Wert | Sprache |
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dc.contributor.author | Elpers, Laura | |
dc.contributor.author | Deiwick, Joerg | |
dc.contributor.author | Hensel, Michael | |
dc.date.accessioned | 2023-02-17T11:31:26Z | - |
dc.date.available | 2023-02-17T11:31:26Z | - |
dc.date.issued | 2022 | |
dc.identifier.uri | http://osnascholar.ub.uni-osnabrueck.de/handle/unios/65261 | - |
dc.description.abstract | Salmonella enterica serovar Typhimurium (STM) is a major cause of gastroenteritis and transmitted by consumption of contaminated food. STM is associated to food origi-nating from animals (pork, chicken, eggs) or plants (veg-etables, fruits, nuts, and herbs). Infection of warm-blooded mammalian hosts by STM and the underlying complex regulatory network of virulence gene expression depend on various environmental conditions encountered in hosts. However, less is known about the proteome and possible regulatory networks for gene expression of STM outside the preferred host. Nutritional limitations and changes in temperature are the most obvious stresses outside the native host. Thus, we analyzed the proteome profile of STM grown in rich medium (LB medium) or minimal me-dium (PCN medium) at temperatures ranging from 8 degrees C to 37 degrees C. LB medium mimics the nutritional rich environment inside the host, whereas minimal PCN medium represents nutritional limitations outside the host, found during growth of fresh produce (field conditions). Further, the range of temperatures analyzed reflects conditions within natural hosts (37 degrees C), room temperature (20 degrees C), during growth under agricultural conditions (16 degrees C and 12 degrees C), and during food storage (8 degrees C). Implications of altered nutrient availability and growth temperature on STM proteomes were analyzed by HPLC/MS-MS and label-free quantification. Our study provides first insights into the complex adaptation of STM to various environmental temperatures, which allows STM not only to infect mammalian hosts but also to enter new infection routes that have been poorly studied so far. With the present dataset, global virulence factors, their impact on infection routes, and potential anti-infective strategies can now be investigated in detail. Especially, we were able to demonstrate functional flagella at 12 degrees C growth temperature for STM with an altered motility behavior. | |
dc.description.sponsorship | [2813HS027 ?]; Acknowledgments - This work was supported by the Bun- desanstalt f?r Landwirtschaft und Ern?hrung (BLE, grant 2813HS027 ?PlantInfect?) . We like to thank Dr Stefan Walter (Universit?t Osnabr?ck, CellNanOs, service unit for mass spectrometry) for the proteomic analyses and support with data analyses. We are grateful to Ursula Krehe and Monika Nietschke for technical support. Further, we like to especially thank Dr Janina Noster for support in strain generation and proteomic data analyses and interpretation. | |
dc.language.iso | en | |
dc.publisher | ELSEVIER | |
dc.relation.ispartof | MOLECULAR & CELLULAR PROTEOMICS | |
dc.subject | Biochemical Research Methods | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | ESCHERICHIA-COLI | |
dc.subject | EXPRESSION | |
dc.subject | GENES | |
dc.subject | H-NS | |
dc.subject | INDUCTION | |
dc.subject | INTEGRATION | |
dc.subject | INTESTINAL COLONIZATION | |
dc.subject | PATHOGENICITY ISLAND 1 | |
dc.subject | STATIONARY-PHASE | |
dc.subject | VIRULENCE | |
dc.title | Effect of Environmental Temperatures on Proteome Composition of Salmonella enterica Serovar Typhimurium | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.mcpro.2022.100265 | |
dc.identifier.isi | ISI:000853691300003 | |
dc.description.volume | 21 | |
dc.description.issue | 8 | |
dc.contributor.orcid | 0000-0001-6604-6253 | |
dc.identifier.eissn | 1535-9484 | |
dc.publisher.place | RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS | |
dcterms.isPartOf.abbreviation | Mol. Cell. Proteomics | |
dcterms.oaStatus | gold, Green Published | |
local.import.remains | affiliations : University Osnabruck; University Osnabruck | |
local.import.remains | web-of-science-index : Science Citation Index Expanded (SCI-EXPANDED) | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0001-6604-6253 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | HeMi480 | - |
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geprüft am 09.06.2024