DC Field | Value | Language |
dc.contributor.author | Gao, Jieqiong | |
dc.contributor.author | Nicastro, Raffaele | |
dc.contributor.author | Peli-Gulli, Marie-Pierre | |
dc.contributor.author | Grziwa, Sophie | |
dc.contributor.author | Chen, Zilei | |
dc.contributor.author | Kurre, Rainer | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | De Virgilio, Claudio | |
dc.contributor.author | Froehlich, Florian | |
dc.contributor.author | Ungermann, Christian | |
dc.date.accessioned | 2023-02-17T11:34:23Z | - |
dc.date.available | 2023-02-17T11:34:23Z | - |
dc.date.issued | 2022 | |
dc.identifier.issn | 0021-9525 | |
dc.identifier.uri | http://osnascholar.ub.uni-osnabrueck.de/handle/unios/65428 | - |
dc.description.abstract | The endomembrane system of eukaryotic cells is essential for cellular homeostasis during growth and proliferation. Previous work showed that a central regulator of growth, namely the target of rapamycin complex 1 (TORC1), binds both membranes of vacuoles and signaling endosomes (SEs) that are distinct from multivesicular bodies (MVBs). Interestingly, the endosomal TORC1, which binds membranes in part via the EGO complex, critically defines vacuole integrity. Here, we demonstrate that SEs form at a branch point of the biosynthetic and endocytic pathways toward the vacuole and depend on MVB biogenesis. Importantly, function of the HOPS tethering complex is essential to maintain the identity of SEs and proper endosomal and vacuolar TORC1 activities. In HOPS mutants, the EGO complex redistributed to the Golgi, which resulted in a partial mislocalization of TORC1. Our study uncovers that SE function requires a functional HOPS complex and MVBs, suggesting a tight link between trafficking and signaling along the endolysosomal pathway. | |
dc.description.sponsorship | Deutsche Forschungsgemeinschaft [UN111/10-2, PI 405/14-1, Sonderforschungsbereich 944]; Swiss National Science Foundation [310030_184671]; This work was funded by the Deutsche Forschungsgemeinschaft (UN111/10-2 to C. Ungermann), imaging facility iBiOs (PI 405/14-1 to J. Piehler and R. Kurre), the Sonderforschungsbereich 944 (project P11 to C. Ungermann, project P20 to F. Frohlich, P8 to J. Piehler, and Z to R. Kurre), and the Swiss National Science Foundation (310030_184671 to C. De Virgilio). | |
dc.language.iso | en | |
dc.publisher | ROCKEFELLER UNIV PRESS | |
dc.relation.ispartof | JOURNAL OF CELL BIOLOGY | |
dc.subject | BINDING | |
dc.subject | Cell Biology | |
dc.subject | COMPARTMENTS | |
dc.subject | CORVET COMPLEX | |
dc.subject | FUSION | |
dc.subject | MEMBRANE | |
dc.subject | PHOSPHOINOSITIDES | |
dc.subject | RAB GTPASES | |
dc.subject | TRANSPORT | |
dc.subject | VPS PROTEIN COMPLEX | |
dc.subject | YEAST SACCHAROMYCES-CEREVISIAE | |
dc.title | The HOPS tethering complex is required to maintain signaling endosome identity and TORC1 activity | |
dc.type | journal article | |
dc.identifier.doi | 10.1083/jcb.202109084 | |
dc.identifier.isi | ISI:000868312600001 | |
dc.description.volume | 221 | |
dc.description.issue | 5 | |
dc.contributor.orcid | 0000-0002-5223-6547 | |
dc.contributor.orcid | 0000-0002-5420-2228 | |
dc.contributor.orcid | 0000-0001-8826-4323 | |
dc.contributor.orcid | 0000-0002-6872-6567 | |
dc.contributor.orcid | 0000-0002-2143-2270 | |
dc.contributor.orcid | 0000-0001-8307-2189 | |
dc.identifier.eissn | 1540-8140 | |
dc.publisher.place | 950 THIRD AVE, 2ND FLR, NEW YORK, NY 10022 USA | |
dcterms.isPartOf.abbreviation | J. Cell Biol. | |
dcterms.oaStatus | Green Published, hybrid | |
local.import.remains | affiliations : University Osnabruck; University of Fribourg; University Osnabruck; University Osnabruck; University Osnabruck | |
local.import.remains | web-of-science-index : Science Citation Index Expanded (SCI-EXPANDED) | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0002-6872-6567 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.orcid | 0000-0001-8307-2189 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | KuRa617 | - |
crisitem.author.netid | PiJa938 | - |
crisitem.author.netid | FrFl166 | - |
crisitem.author.netid | UnCh999 | - |