Cvm1 is a component of multiple vacuolar contact sites required for sphingolipid homeostasis
DC Element | Wert | Sprache |
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dc.contributor.author | Bisinski, Daniel D. | |
dc.contributor.author | Castro, Ines Gomes | |
dc.contributor.author | Mari, Muriel | |
dc.contributor.author | Walter, Stefan | |
dc.contributor.author | Frohlich, Florian | |
dc.contributor.author | Schuldiner, Maya | |
dc.contributor.author | Montoro, Ayelen Gonzalez | |
dc.date.accessioned | 2023-02-17T11:35:39Z | - |
dc.date.available | 2023-02-17T11:35:39Z | - |
dc.date.issued | 2022 | |
dc.identifier.issn | 0021-9525 | |
dc.identifier.uri | http://osnascholar.ub.uni-osnabrueck.de/handle/unios/65523 | - |
dc.description.abstract | Membrane contact sites are specialized platforms formed between most organelles that enable them to exchange metabolites and influence the dynamics of each other. The yeast vacuole is a degradative organelle equivalent to the lysosome in higher eukaryotes with important roles in ion homeostasis and metabolism. Using a high-content microscopy screen, we identified Ymr160w (Cvm1, for contact of the vacuole membrane 1) as a novel component of three different contact sites of the vacuole: with the nuclear endoplasmic reticulum, the mitochondria, and the peroxisomes. At the vacuole-mitochondria contact site, Cvm1 acts as a tether independently of previously known tethers. We show that changes in Cvm1 levels affect sphingolipid homeostasis, altering the levels of multiple sphingolipid classes and the response of sphingolipid-sensing signaling pathways. Furthermore, the contact sites formed by Cvm1 are induced upon a decrease in sphingolipid levels. Altogether, our work identifies a novel protein that forms multiple contact sites and supports a role of lysosomal contacts in sphingolipid homeostasis. | |
dc.description.sponsorship | Schering and Fritz Thyssen foundations [20.18.0.029MN]; Deutsche Forchungsgemeinschaft [SFB944, SFB1190]; Volkswagen Foundation Life Grant [93092MS]; European Molecular Biology Organization Advanced Long-term Fellowship [aALTF 609-2018]; Dutch Research Council [ALWOP.355]; European Molecular Biology Organization Long-term Fellowship [ALTF-580-2017]; This work was funded by a Young Investigator Fund grant from the Schering and Fritz Thyssen foundations (20.18.0.029MN) and a Deutsche Forchungsgemeinschaft Grant within the SFB944 to A. Gonzalez Montoro, as well as by a Deutsche Forchungsgemeinschaft grant within the SFB1190 and a Volkswagen Foundation Life Grant (93092MS) to M. Schuldiner. A. Gonzalez Montoro is a recipient of a European Molecular Biology Organization Advanced Long-term Fellowship (aALTF 609-2018). M. Schuldiner is an incumbent of the Dr. Gilbert Omenn and Martha Darling Professorial Chair in Molecular Genetics. M. Mari is supported by an ALW Open Programme grant (ALWOP.355) of the Dutch Research Council. I. Gomes Castro is the recipient of a European Molecular Biology Organization Long-term Fellowship (ALTF-580-2017). | |
dc.language.iso | en | |
dc.publisher | ROCKEFELLER UNIV PRESS | |
dc.relation.ispartof | JOURNAL OF CELL BIOLOGY | |
dc.subject | Cell Biology | |
dc.subject | ER-MITOCHONDRIA | |
dc.subject | GOLGI | |
dc.subject | INTERFACE | |
dc.subject | JUNCTIONS | |
dc.subject | PROTEIN TOPOLOGY | |
dc.subject | SACCHAROMYCES-CEREVISIAE | |
dc.subject | STRESS | |
dc.subject | TETHER | |
dc.subject | YEAST | |
dc.title | Cvm1 is a component of multiple vacuolar contact sites required for sphingolipid homeostasis | |
dc.type | journal article | |
dc.identifier.doi | 10.1083/jcb.202103048 | |
dc.identifier.isi | ISI:000868315600001 | |
dc.description.volume | 221 | |
dc.description.issue | 8 | |
dc.contributor.orcid | 0000-0002-2677-6945 | |
dc.contributor.orcid | 0000-0003-4669-985X | |
dc.contributor.orcid | 0000-0001-6762-5070 | |
dc.contributor.orcid | 0000-0002-6978-8284 | |
dc.contributor.orcid | 0000-0001-8307-2189 | |
dc.contributor.orcid | 0000-0001-9947-115X | |
dc.identifier.eissn | 1540-8140 | |
dc.publisher.place | 950 THIRD AVE, 2ND FLR, NEW YORK, NY 10022 USA | |
dcterms.isPartOf.abbreviation | J. Cell Biol. | |
local.import.remains | affiliations : University Osnabruck; Weizmann Institute of Science; University of Groningen; University Osnabruck | |
local.import.remains | web-of-science-index : Science Citation Index Expanded (SCI-EXPANDED) | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0001-8307-2189 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | FrFl166 | - |
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geprüft am 29.05.2024