Mechanisms of Formation, Structure, and Dynamics of Lipoprotein Discs Stabilized by Amphiphilic Copolymers: A Comprehensive Review
DC Element | Wert | Sprache |
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dc.contributor.author | Orekhov, Philipp S. | |
dc.contributor.author | Bozdaganyan, Marine E. | |
dc.contributor.author | Voskoboynikova, Natalia | |
dc.contributor.author | Mulkidjanian, Armen Y. | |
dc.contributor.author | Karlova, Maria G. | |
dc.contributor.author | Yudenko, Anna | |
dc.contributor.author | Remeeva, Alina | |
dc.contributor.author | Ryzhykau, Yury L. | |
dc.contributor.author | Gushchin, Ivan | |
dc.contributor.author | Gordeliy, Valentin I. | |
dc.contributor.author | Sokolova, Olga S. | |
dc.contributor.author | Steinhoff, Heinz-Juergen | |
dc.contributor.author | Kirpichnikov, Mikhail P. | |
dc.contributor.author | Shaitan, Konstantin V. | |
dc.date.accessioned | 2023-02-17T11:36:55Z | - |
dc.date.available | 2023-02-17T11:36:55Z | - |
dc.date.issued | 2022 | |
dc.identifier.uri | http://osnascholar.ub.uni-osnabrueck.de/handle/unios/65639 | - |
dc.description.abstract | Amphiphilic copolymers consisting of alternating hydrophilic and hydrophobic units account for a major recent methodical breakthrough in the investigations of membrane proteins. Styrene-maleic acid (SMA), diisobutylene-maleic acid (DIBMA), and related copolymers have been shown to extract membrane proteins directly from lipid membranes without the need for classical detergents. Within the particular experimental setup, they form disc-shaped nanoparticles with a narrow size distribution, which serve as a suitable platform for diverse kinds of spectroscopy and other biophysical techniques that require relatively small, homogeneous, water-soluble particles of separate membrane proteins in their native lipid environment. In recent years, copolymer-encased nanolipoparticles have been proven as suitable protein carriers for various structural biology applications, including cryo-electron microscopy (cryo-EM), small-angle scattering, and conventional and single-molecule X-ray diffraction experiments. Here, we review the current understanding of how such nanolipoparticles are formed and organized at the molecular level with an emphasis on their chemical diversity and factors affecting their size and solubilization efficiency. | |
dc.description.sponsorship | Russian Science Foundation [19-74-30003]; Russian Foundation for Basic Research [18-02-40010, 19-29-12022]; German Research Foundation (DFG) [STE640/15]; Russian Ministry of Science and Higher Education [075-15-2021-1354, 075-00337-20-03, FSMG-2020-0003]; Interdisciplinary Scientific and Educational Schools of Moscow University ``Molecular Technologies of the Living Systems and Synthetic Biology''; Interdisciplinary Scientific and Educational Schools of Moscow University ``Brain, cognitive systems, artificial intelligence''; German Academic Exchange Service (DAAD); Ostpartnerschaftenprogramm; This research was funded by the Russian Science Foundation grant No. 19-74-30003 to M.P.K., Russian Foundation for Basic Research No. 19-29-12022 to V.I.G. (X-ray crystallography), Russian Foundation for Basic Research grant No. 18-02-40010 to K.V.S. (SMA MD simulations), German Research Foundation (DFG, STE640/15) to H.J.S. and the Russian Ministry of Science and Higher Education grants No. 075-15-2021-1354 to M.P.K. and K.V.S. (DIBMA MD simulations) and No. 075-00337-20-03, project FSMG-2020-0003 to A.Yu. and Yu.R. (small-angle scattering). This research has been non-profit supported by the Interdisciplinary Scientific and Educational Schools of Moscow University ``Molecular Technologies of the Living Systems and Synthetic Biology'' and ``Brain, cognitive systems, artificial intelligence''. A.Y.M., K.V.S. and H.J.S. would like to thank the German Academic Exchange Service (DAAD) and its Ostpartnerschaftenprogramm for support. Also, O.S.S. thanks the unique equipment setup ``3D-EMS'' of Lomonosov Moscow State University. | |
dc.language.iso | en | |
dc.publisher | MDPI | |
dc.relation.ispartof | NANOMATERIALS | |
dc.subject | amphiphilic copolymers | |
dc.subject | BILAYER NANODISCS | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | DETERGENT-FREE ISOLATION | |
dc.subject | DIBMA | |
dc.subject | INTEGRAL MEMBRANE-PROTEINS | |
dc.subject | LIPID NANODISC FORMATION | |
dc.subject | lipodiscs | |
dc.subject | LIPODISQ NANOPARTICLES | |
dc.subject | MALEIC ACID COPOLYMER | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | nanolipoparticles | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | PH | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | POLYMER | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | SMA | |
dc.subject | SMALL-ANGLE SCATTERING | |
dc.subject | SOLUBILIZATION | |
dc.subject | structural biology | |
dc.title | Mechanisms of Formation, Structure, and Dynamics of Lipoprotein Discs Stabilized by Amphiphilic Copolymers: A Comprehensive Review | |
dc.type | review | |
dc.identifier.doi | 10.3390/nano12030361 | |
dc.identifier.isi | ISI:000757348100001 | |
dc.description.volume | 12 | |
dc.description.issue | 3 | |
dc.contributor.orcid | 0000-0003-4078-4762 | |
dc.contributor.orcid | 0000-0002-5348-6070 | |
dc.contributor.orcid | 0000-0002-5888-0157 | |
dc.contributor.orcid | 0000-0003-3719-1464 | |
dc.contributor.orcid | 0000-0003-3977-3679 | |
dc.contributor.orcid | 0000-0002-3115-0015 | |
dc.contributor.researcherid | G-7794-2016 | |
dc.contributor.researcherid | V-5214-2017 | |
dc.contributor.researcherid | K-9197-2015 | |
dc.contributor.researcherid | H-3791-2014 | |
dc.identifier.eissn | 2079-4991 | |
dc.publisher.place | ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND | |
dcterms.isPartOf.abbreviation | Nanomaterials | |
dcterms.oaStatus | gold, Green Published | |
local.import.remains | affiliations : Lomonosov Moscow State University; Sechenov First Moscow State Medical University; Russian Academy of Sciences; N.N. Semenov Federal Research Centre for Chemical Physics, Russian Academy of Sciences; University Osnabruck; Lomonosov Moscow State University; Lomonosov Moscow State University; Moscow Institute of Physics & Technology; Helmholtz Association; Research Center Julich; UDICE-French Research Universities; Communaute Universite Grenoble Alpes; Universite Grenoble Alpes (UGA); CEA; Centre National de la Recherche Scientifique (CNRS); Helmholtz Association; Research Center Julich; Russian Academy of Sciences; Pushchino Scientific Center for Biological Research (PSCBI) of the Russian Academy of Sciences; Institute of Bioorganic Chemistry of the Russian Academy of Sciences | |
local.import.remains | web-of-science-index : Science Citation Index Expanded (SCI-EXPANDED) | |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.orcid | 0000-0003-2317-0144 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | VoNa568 | - |
crisitem.author.netid | StHe633 | - |
crisitem.author.netid | ShKo001 | - |
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