ATP SYNTHESIS ENERGIZED BY DELTA-PNA AND DELTA-PHI IN PROTEOLIPOSOMES CONTAINING THE F(0)F(1)-ATPASE FROM PROPIONIGENIUM-MODESTUM

Autor(en): DMITRIEV, O
DECKERSHEBESTREIT, G
ALTENDORF, K 
Stichwörter: ADENOSINE-TRIPHOSPHATASE; BETA-SUBUNIT; Biochemistry & Molecular Biology; DECOMPOSE FATTY-ACIDS; ENZYME; ESCHERICHIA-COLI; GEN-NOV; ION; RECONSTITUTION; SULFATE-REDUCING BACTERIA; SYNTHASE
Erscheinungsdatum: 1993
Herausgeber: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Enthalten in: JOURNAL OF BIOLOGICAL CHEMISTRY
Band: 268
Ausgabe: 20
Startseite: 14776
Seitenende: 14780
Zusammenfassung: 
After incorporation of the purified Na+-translocating F0F1-ATPase from Propionigenium modestum into preformed phospholipid vesicles the synthesis of ATP from ADP and inorganic phosphate could be observed under conditions where a valinomycin-mediated K+ diffusion potential (DELTAphi) and/or a Na+ concentration gradient (DELTApNa) were imposed. This reaction was not inhibited by the protonophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). Furthermore, the DELTApNa-driven ATP synthesis was stimulated by FCCP. In contrast, the addition of the Na+/H+ antiporter monensin or of the F0F1 inhibitors N,N-dicyclohexylcarbodiimide and venturicidin abolished the synthesis of ATP completely. Finally, DELTApNa alone was able to elicit ATP synthesis, when a Na+ concentration gradient of sufficient magnitude was applied. In this case ATP synthesis occurred above a threshold level of approximately 120 mV and, furthermore, DELTAphi and DELTApNa appear to be equivalent as driving forces for this process. Therefore, the data provide firm evidence for the concept that DELTAmu''Na+ is the primary driving force for the synthesis of ATP in P. modestum.
ISSN: 00219258

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