Analysis of the protein composition of the spindle pole body during sporulation in Ashbya gossypii
DC Element | Wert | Sprache |
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dc.contributor.author | Wabner, Dario | |
dc.contributor.author | Overhageboeck, Tom | |
dc.contributor.author | Nordmann, Doris | |
dc.contributor.author | Kronenberg, Julia | |
dc.contributor.author | Kramer, Florian | |
dc.contributor.author | Schmitz, Hans-Peter | |
dc.date.accessioned | 2021-12-23T16:04:58Z | - |
dc.date.available | 2021-12-23T16:04:58Z | - |
dc.date.issued | 2019 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/6711 | - |
dc.description.abstract | The spores of fungi come in a wide variety of forms and sizes, highly adapted to the route of dispersal and to survival under specific environmental conditions. The ascomycete Ashbya gossypii produces needle shaped spores with a length of 30 pm and a diameter of 1 pm. Formation of these spores relies on actin and actin regulatory proteins and is, therefore, distinct from the minor role that actin plays for spore formation in Saccharomyces cerevisiae. Using in vivo FRET-measurements of proteins labeled with fluorescent proteins, we investigate how the formin AgBnr2, a protein that promotes actin polymerization, integrates into the structure of the spindle pole body during sporulation. We also investigate the role of the A. gossypii homologs to the S. cerevisiae meiotic outer plaque proteins Spo74, Mpc54 and Ady4 for sporulation in A. gossypii. We found highest FRET of AgBnr2 with AgSpo74. Further experiments indicated that AgSpo74 is a main factor for targeting AgBnr2 to the spindle pole body. In agreement with these results, the Agspo74 deletion mutant produces no detectable spores, whereas deletion of Agmpc54 only has an effect on spore length and deletion of Agady4 has no detectable sporulation phenotype. Based on this study and in relation to previous results we suggest a model where AgBnr2 resides within an analogous structure to the meiotic outer plaque of S. cerevisiae. There it promotes formation of actin cables important for shaping the needle shaped spore structure. | |
dc.description.sponsorship | Deutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [SCHM 2388/2-1]; Osnabruck University [SCHM 2388/2-1]; This work was supported by grant SCHM 2388/2-1 from the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publishing Fund of Osnabruck University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; This work was supported by grant SCHM 2388/2-1 from the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publishing Fund of Osnabruck University. We thank Sandra Bartels and Bernadette Sander-Turgut for excellent technical support, Lucille Schmieding for help with editing the manuscript and Jurgen J. Heinisch for constant support. | |
dc.language.iso | en | |
dc.publisher | PUBLIC LIBRARY SCIENCE | |
dc.relation.ispartof | PLOS ONE | |
dc.subject | ACTIN | |
dc.subject | MEIOSIS | |
dc.subject | Multidisciplinary Sciences | |
dc.subject | ORGANIZATION | |
dc.subject | PROSPORE MEMBRANE | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | YEAST | |
dc.title | Analysis of the protein composition of the spindle pole body during sporulation in Ashbya gossypii | |
dc.type | journal article | |
dc.identifier.doi | 10.1371/journal.pone.0223374 | |
dc.identifier.isi | ISI:000532398600057 | |
dc.description.volume | 14 | |
dc.description.issue | 10 | |
dc.contributor.orcid | 0000-0001-5449-4593 | |
dc.publisher.place | 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA | |
dcterms.isPartOf.abbreviation | PLoS One | |
dcterms.oaStatus | gold, Green Submitted, Green Published | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0001-5449-4593 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | WaDa541 | - |
crisitem.author.netid | NoDo739 | - |
crisitem.author.netid | ScHa130 | - |
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geprüft am 13.05.2024