ISOZYMES AND GENERAL PROTEIN-PATTERNS AS TAXONOMIC MARKERS IN THE TAXON NEPHTYIDAE (ANNELIDA-POLYCHAETA)

DC FieldValueLanguage
dc.contributor.authorSCHMIDT, H
dc.contributor.authorWESTHEIDE, W
dc.date.accessioned2021-12-23T16:05:10Z-
dc.date.available2021-12-23T16:05:10Z-
dc.date.issued1994
dc.identifier.issn00253162
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/6820-
dc.description.abstractSeven Nephtys species and one species of the genus Aglaophamus (collected from different European tidal and subtidal locations between 1989 and 1991) were compared with respect to six isozyme systems (alpha-amylase, esterase, hexokinase, malate dehydrogenase, malic enzyme, phosphoglucoseisomerase) as well as with respect to general protein patterns with non-specific staining. The proteins were obtained from the tissue (proboscis, individual segments) of single individuals, the species of which had previously been accurately determined, and were separated by isoelectric focusing (IEF) in polyacrylamide gels. The enzymes were identified by their specific catalytic activities, and the general proteins were visualized by silver staining. All the isozymes proved to be monomorphic within each of the various polychaete populations. With a single exception, it was impossible to distinguish geographically separated populations of the same species, because the band patterns were completely consistent within the species. The individual enzymes varied in their suitability for species differentiation. Only with respect to esterase and alpha-amylase could all species be distinguished; the other enzymes studied were identical in morphologically similar species. In contrast, each species could be identified by its general protein pattern, although some species differed from others in the position of a few bands only. Individuals of N. hombergii, N. caeca and N. cirrosa with abnormally shaped parapodia, as well as juvenile nephtyids, could be unequivocally assigned to their respective species by IEF. Only in the case of N. longosetosa were two morphological variants found to differ in their electrophoretic characteristics; one of them is interpreted as a new species, not previously described.
dc.language.isoen
dc.publisherSPRINGER VERLAG
dc.relation.ispartofMARINE BIOLOGY
dc.subjectGEL-ELECTROPHORESIS
dc.subjectIDENTIFICATION
dc.subjectKEY
dc.subjectMarine & Freshwater Biology
dc.subjectNATURAL-POPULATIONS
dc.subjectNORTHERN EUROPE
dc.subjectPHYLLODOCIDA
dc.subjectREDESCRIPTION
dc.subjectSPECIES OLIGOCHAETA
dc.titleISOZYMES AND GENERAL PROTEIN-PATTERNS AS TAXONOMIC MARKERS IN THE TAXON NEPHTYIDAE (ANNELIDA-POLYCHAETA)
dc.typejournal article
dc.identifier.doi10.1007/BF00350103
dc.identifier.isiISI:A1994NK27500004
dc.description.volume119
dc.description.issue1
dc.description.startpage31
dc.description.endpage38
dc.publisher.place175 FIFTH AVE, NEW YORK, NY 10010
dcterms.isPartOf.abbreviationMar. Biol.
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