Electrostatically Controlled Quantum Dot Monofunctionalization for Interrogating the Dynamics of Protein Complexes in Living Cells
DC Element | Wert | Sprache |
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dc.contributor.author | You, Changjiang | |
dc.contributor.author | Wilmes, Stephan | |
dc.contributor.author | Richter, Christian P. | |
dc.contributor.author | Beutel, Oliver | |
dc.contributor.author | Lisse, Domenik | |
dc.contributor.author | Piehler, Jacob | |
dc.date.accessioned | 2021-12-23T16:05:15Z | - |
dc.date.available | 2021-12-23T16:05:15Z | - |
dc.date.issued | 2013 | |
dc.identifier.issn | 15548929 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/6879 | - |
dc.description.abstract | Quantum dots (QD) are powerful labels for probing diffusion and interaction dynamics of proteins on the single molecule level in living cells. Protein cross-linking due to multifunctional QD strongly affects these properties. This becomes particularly critical when labeling interaction partners with QDs for interrogating the dynamics of complexes. We have here implemented a generic method for QD monofunctionalization based on electrostatic repulsion of a highly negatively charged peptide carrier. On the basis of this method, monobiotinylated QDs were prepared with high yield as confirmed by single molecule assays. These QDs were successfully employed for probing the assembly and diffusion dynamics of binary and ternary cytokine-receptor complexes on the surface of living cells by dual color single QD tracking. Thus, sequential and dynamic recruitment of the type I interferon receptor subunits by the ligand could be observed. | |
dc.description.sponsorship | DFGGerman Research Foundation (DFG)European Commission [P1405-6, SFB 944]; European Community's Seventh Framework Programme (FP7)European Commission [223608]; We thank G. Hikade and H. Kenneweg for technical support. Plasmids for producing mSAV and bSAV as well as BirA were kindly provided by A. Ting, MIT. This work was supported by the DFG (P1405-6 and SFB 944) and the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 223608 (IFNaction). | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | ACS CHEMICAL BIOLOGY | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | FUNCTIONALIZATION | |
dc.subject | HISTIDINE-TAGGED PROTEINS | |
dc.subject | I INTERFERON RECEPTOR | |
dc.subject | LIVE CELLS | |
dc.subject | MICROSCOPY | |
dc.subject | NANOPARTICLES | |
dc.subject | ORGANIZATION | |
dc.subject | PLASMA-MEMBRANE | |
dc.subject | SIGNAL-TRANSDUCTION | |
dc.subject | SINGLE-MOLECULE TRACKING | |
dc.title | Electrostatically Controlled Quantum Dot Monofunctionalization for Interrogating the Dynamics of Protein Complexes in Living Cells | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/cb300543t | |
dc.identifier.isi | ISI:000315253700007 | |
dc.description.volume | 8 | |
dc.description.issue | 2 | |
dc.description.startpage | 320 | |
dc.description.endpage | 326 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.orcid | 0000-0002-4112-710X | |
dc.contributor.researcherid | L-3901-2014 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | ACS Chem. Biol. | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | YoCh745 | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 10.05.2024