The terminal reaction cascade of water oxidation: Proton and oxygen release

DC FieldValueLanguage
dc.contributor.authorClausen, Juergen
dc.contributor.authorJunge, Wolfgang
dc.date.accessioned2021-12-23T16:05:18Z-
dc.date.available2021-12-23T16:05:18Z-
dc.date.issued2008
dc.identifier.issn00052728
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/6904-
dc.description.abstractIn cyanobacteria, algae and plants Photosystem II produces the oxygen we breathe. Driven and clocked by light quanta, the catalytic Mn4Ca-tyrosine centre accumulates four oxidising equivalents before it abstracts four electrons from water, liberating dioxygen and protons. Aiming at intermediates of the terminal four-electron cascade, we previously have suppressed this reaction by elevating the oxygen pressure, thereby stabilising one redox intermediate. Here, we established a similar suppression by increasing the proton concentration. Data were analysed in terms of only one (peroxy) redox intermediate between the fourfold oxidised Mn4Ca-tyrosine centre and oxygen release. The surprising result was that the release into the bulk of one proton per dioxygen is linked to the first and rate-limiting electron transfer in the cascade rather than to the second which produces free oxygen. The penultimate intermediate might thus be conceived as a fully deprotonated peroxy-moiety. (C) 2008 Elsevier B.V. All rights reserved.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [Ju97/15, 1-4]; Fonds der Chemischen IndustrieFonds der Chemischen IndustrieEuropean Commission; Land Niedersachsen; The authors are grateful to Hella Kenneweg for the excellent technical assistance, Holger Heine for the advice on the construction of the pressure cell and Prof. Rick Debus for the cooperation on Synechocystis.; This work was financially supported by the Deutsche Forschungsgemeinschaft (Ju97/15, 1-4), the Fonds der Chemischen Industrie and the Land Niedersachsen.
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofBIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCHARGE RECOMBINATION
dc.subjectCHLOROPHYLL FLUORESCENCE
dc.subjectDONOR SIDE
dc.subjectELECTRON-TRANSFER
dc.subjectFTIR DIFFERENCE SPECTROSCOPY
dc.subjectIntermediate
dc.subjectOxygen
dc.subjectPH-DEPENDENCE
dc.subjectPhotosynthesis
dc.subjectPhotosystem II
dc.subjectPHOTOSYSTEM-II PARTICLES
dc.subjectPROTOLYTIC REACTIONS
dc.subjectProton release
dc.subjectSYNECHOCYSTIS SP PCC-6803
dc.subjectTYROSINE Y-Z
dc.subjectWater oxidation
dc.titleThe terminal reaction cascade of water oxidation: Proton and oxygen release
dc.typejournal article
dc.identifier.doi10.1016/j.bbabio.2008.06.009
dc.identifier.isiISI:000260284300009
dc.description.volume1777
dc.description.issue10
dc.description.startpage1311
dc.description.endpage1318
dc.identifier.eissn00063002
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationBiochim. Biophys. Acta-Bioenerg.
dcterms.oaStatusBronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidJuWo587-
Show simple item record

Google ScholarTM

Check

Altmetric