Depalmitoylation of Ykt6 prevents its entry into the multivesicular body pathway

DC FieldValueLanguage
dc.contributor.authorMeiringer, Christoph T. A.
dc.contributor.authorAuffarth, Kathrin
dc.contributor.authorHou, Haitong
dc.contributor.authorUngermann, Christian
dc.date.accessioned2021-12-23T16:05:18Z-
dc.date.available2021-12-23T16:05:18Z-
dc.date.issued2008
dc.identifier.issn13989219
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/6905-
dc.description.abstractThe dually lipidated SNARE Ykt6 is found on intracellular membranes and in the cytosol. In this study, we show that Ykt6 localizes to the Golgi as well as endosomal and vacuolar membranes in vivo. The ability of Ykt6 to cycle between the cytosol and the membranes depends on the intramolecular interaction of the N-terminal longin and C-terminal SNARE domains and not on either domain alone. A mutant deficient in this interaction accumulates on membranes and - in contrast to the wild-type protein - does not get released from vacuoles. Our data also indicate that Ykt6 is a substrate of the DHHC (Asp-His-His-Cys) acyltransferase network. Overexpression of the vacuolar acyltransferase Pfa3 drives the F42S mutant not only to the vacuole but also into the vacuolar lumen. Thus, depalmitoylation and release of Ykt6 are needed for its recycling and to circumvent its entry into the endosomal multivesicular body pathway.
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofTRAFFIC
dc.subjectCell Biology
dc.subjectCIS-SNARE
dc.subjectCOMPLEXES
dc.subjectEARLY-STAGE
dc.subjectfusion
dc.subjectMECHANISM
dc.subjectMEMBRANE-FUSION
dc.subjectPROTEIN PALMITOYLATION
dc.subjectprotein sorting
dc.subjectR-SNARE
dc.subjectSNARE
dc.subjectSNARE YKT6
dc.subjectSPECIFICITY
dc.subjectTRANSPORT
dc.subjectvacuole
dc.subjectYkt6
dc.titleDepalmitoylation of Ykt6 prevents its entry into the multivesicular body pathway
dc.typejournal article
dc.identifier.doi10.1111/j.1600-0854.2008.00778.x
dc.identifier.isiISI:000258446200011
dc.description.volume9
dc.description.issue9
dc.description.startpage1510
dc.description.endpage1521
dc.contributor.orcid0000-0003-4493-9645
dc.contributor.researcheridE-8416-2013
dc.contributor.researcheridD-5188-2019
dc.identifier.eissn16000854
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationTraffic
dcterms.oaStatusBronze
crisitem.author.netidUnCh999-
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