Interaction of viologens with nucleic acid G-tetrades
Autor(en): | Asaftei, Simona Rosemeyer, Helmut Walder, Lorenz |
Stichwörter: | Chemistry; Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science; Materials Science, Multidisciplinary | Erscheinungsdatum: | 2008 | Herausgeber: | AMER CHEMICAL SOC | Journal: | LANGMUIR | Volumen: | 24 | Ausgabe: | 11 | Startseite: | 5641 | Seitenende: | 5643 | Zusammenfassung: | The interaction between the tetrade-forming oligonucleotide 5'-d(T(4)G(4)T(4)) and monoalkylated bipyridinium salts, such as 1-ethyl-4-pyridin-4-ylpyridinium bromide, is reported. The oligomer forms tetrades in the presence of K+ ions but not with Li+. Additionally, the interaction of the thrombin-binding aptamer 5'-d(GGTTGGTGTGGTTGG) (TBA) with a dialkylated bipyridinium salt, viologen, was studied by cyclic voltammetry. This was performed either on a TiO2 electrode, derivatized with 3-aminopropyltriethoxysilane (APS), using [Fe(CN)(6)](4-) as a marker ion or without a marker ion on an electrostatically TiO2-bound amino-ferrocenyl derivative. Both experiments proof a strong interaction between the immobilized aptamer and the viologen. Third, the electrochemical response of the specific thrombin binding to the immobilized aptamer was studied. |
ISSN: | 07437463 | DOI: | 10.1021/la704001n |
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