Specific DNA duplex formation at an artificial lipid bilayer: fluorescence microscopy after Sybr Green I staining

DC FieldValueLanguage
dc.contributor.authorWerz, Emma
dc.contributor.authorRosemeyer, Helmut
dc.date.accessioned2021-12-23T16:05:30Z-
dc.date.available2021-12-23T16:05:30Z-
dc.date.issued2014
dc.identifier.issn18605397
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7043-
dc.description.abstractThe article describes the immobilization of different probe oligonucleotides (4, 7, 10) carrying each a racemic mixture of 2,3-bis(hexadecyloxy) propan-1-ol (1a) at the 5'-terminus on a stable artificial lipid bilayer composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). The bilayer separates two compartments (cis/trans channel) of an optical transparent microfluidic sample carrier with perfusion capabilities. Injection of unlabeled target DNA sequences (6, 8, or 9), differing in sequence and length, leads in the case of complementarity to the formation of stable DNA duplexes at the bilayer surface. This could be verified by Sybr Green I double strand staining, followed by incubation periods and thorough perfusions, and was visualized by single molecule fluorescence spectroscopy and microscopy. The different bilayer-immobilized complexes consisting of various DNA duplexes and the fluorescent dye were studied with respect to the kinetics of their formation as well as to their stability against perfusion.
dc.description.sponsorshipIonovation GmbH, Osnabruck, Germany; The authors gratefully acknowledge financial support by the Ionovation GmbH, Osnabruck, Germany, as well as valuable discussions with Prof. Dr. Richard Wagner and Mr. Claudius Walter, University of Osnabruck, Department of Biophysics.
dc.language.isoen
dc.publisherBEILSTEIN-INSTITUT
dc.relation.ispartofBEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
dc.subjectartificial lipid bilayers
dc.subjectCARCINOMA CELLS
dc.subjectChemistry
dc.subjectChemistry, Organic
dc.subjectCOLON
dc.subjectDELIVERY
dc.subjectlipo-oligonucleotide duplexes
dc.subjectMEMBRANES
dc.subjectnucleic acids
dc.subjectNUCLEIC-ACIDS
dc.subjectNUCLEOLIPIDS
dc.subjectSybr Green I
dc.titleSpecific DNA duplex formation at an artificial lipid bilayer: fluorescence microscopy after Sybr Green I staining
dc.typejournal article
dc.identifier.doi10.3762/bjoc.10.240
dc.identifier.isiISI:000342705300001
dc.description.volume10
dc.description.startpage2307
dc.description.endpage2321
dc.publisher.placeTRAKEHNER STRASSE 7-9, FRANKFURT AM MAIN, 60487, GERMANY
dcterms.isPartOf.abbreviationBeilstein J. Org. Chem.
dcterms.oaStatusGreen Published, gold, Green Submitted
crisitem.author.deptInstitut für Chemie neuer Materialien-
crisitem.author.deptidinstitute11-
crisitem.author.parentorgFB 05 - Biologie/Chemie-
crisitem.author.grandparentorgUniversität Osnabrück-
crisitem.author.netidRoHe783-
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