DC Element | Wert | Sprache |
dc.contributor.author | Werz, Emma | |
dc.contributor.author | Rosemeyer, Helmut | |
dc.date.accessioned | 2021-12-23T16:05:30Z | - |
dc.date.available | 2021-12-23T16:05:30Z | - |
dc.date.issued | 2014 | |
dc.identifier.issn | 18605397 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/7043 | - |
dc.description.abstract | The article describes the immobilization of different probe oligonucleotides (4, 7, 10) carrying each a racemic mixture of 2,3-bis(hexadecyloxy) propan-1-ol (1a) at the 5'-terminus on a stable artificial lipid bilayer composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). The bilayer separates two compartments (cis/trans channel) of an optical transparent microfluidic sample carrier with perfusion capabilities. Injection of unlabeled target DNA sequences (6, 8, or 9), differing in sequence and length, leads in the case of complementarity to the formation of stable DNA duplexes at the bilayer surface. This could be verified by Sybr Green I double strand staining, followed by incubation periods and thorough perfusions, and was visualized by single molecule fluorescence spectroscopy and microscopy. The different bilayer-immobilized complexes consisting of various DNA duplexes and the fluorescent dye were studied with respect to the kinetics of their formation as well as to their stability against perfusion. | |
dc.description.sponsorship | Ionovation GmbH, Osnabruck, Germany; The authors gratefully acknowledge financial support by the Ionovation GmbH, Osnabruck, Germany, as well as valuable discussions with Prof. Dr. Richard Wagner and Mr. Claudius Walter, University of Osnabruck, Department of Biophysics. | |
dc.language.iso | en | |
dc.publisher | BEILSTEIN-INSTITUT | |
dc.relation.ispartof | BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY | |
dc.subject | artificial lipid bilayers | |
dc.subject | CARCINOMA CELLS | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Organic | |
dc.subject | COLON | |
dc.subject | DELIVERY | |
dc.subject | lipo-oligonucleotide duplexes | |
dc.subject | MEMBRANES | |
dc.subject | nucleic acids | |
dc.subject | NUCLEIC-ACIDS | |
dc.subject | NUCLEOLIPIDS | |
dc.subject | Sybr Green I | |
dc.title | Specific DNA duplex formation at an artificial lipid bilayer: fluorescence microscopy after Sybr Green I staining | |
dc.type | journal article | |
dc.identifier.doi | 10.3762/bjoc.10.240 | |
dc.identifier.isi | ISI:000342705300001 | |
dc.description.volume | 10 | |
dc.description.startpage | 2307 | |
dc.description.endpage | 2321 | |
dc.publisher.place | TRAKEHNER STRASSE 7-9, FRANKFURT AM MAIN, 60487, GERMANY | |
dcterms.isPartOf.abbreviation | Beilstein J. Org. Chem. | |
dcterms.oaStatus | Green Published, gold, Green Submitted | |
crisitem.author.dept | Institut für Chemie neuer Materialien | - |
crisitem.author.deptid | institute11 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | RoHe783 | - |