Specific DNA Duplex Formation at an Artificial Lipid Bilayer: towards a New DNA Biosensor Technology
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Werz, Emma | |
dc.contributor.author | Korneev, Sergei | |
dc.contributor.author | Montilla-Martinez, Malayko | |
dc.contributor.author | Wagner, Richard | |
dc.contributor.author | Hemmler, Roland | |
dc.contributor.author | Walter, Claudius | |
dc.contributor.author | Eisfeld, Joerg | |
dc.contributor.author | Gall, Karsten | |
dc.contributor.author | Rosemeyer, Helmut | |
dc.date.accessioned | 2021-12-23T16:05:31Z | - |
dc.date.available | 2021-12-23T16:05:31Z | - |
dc.date.issued | 2012 | |
dc.identifier.issn | 16121872 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/7047 | - |
dc.description.abstract | A novel technique is described which comprises a base-specific DNA duplex formation at a lipid bilayer?H2O-phase boundary layer. Two different probes of oligonucleotides both carrying a double-tailed lipid at the 5'-terminus were incorporated into stable artificial lipid bilayers separating two compartments (cis/trans-channel) of an optically transparent microfluidic sample carrier with perfusion capabilities. Both the cis- and trans-channels are filled with saline buffer. Injection of a cyanine-5-labeled target DNA sequence, which is complementary to only one of the oligonucleotide probes, into the cis-channel, followed by a thorough perfusion, leads to an immobilization of the labeled complementary oligonucleotide on the membrane as detected by single-molecule fluorescence spectroscopy and microscopy. In the case of fluorescent but non-complementary DNA sequences, no immobilized fluorescent oligonucleotide duplex could be detected on the membrane. This clearly verifies a specific duplex formation at the membrane interface. | |
dc.description.sponsorship | Bundesministerium fur Wirtschaft (FKZ) [KF 2369401 S B9, KF 2369501 S B9]; The authors thank the Bundesministerium fur Wirtschaft for financial support (FKZ: KF 2369401 S B9 and KF 2369501 S B9). | |
dc.language.iso | en | |
dc.publisher | WILEY-V C H VERLAG GMBH | |
dc.relation.ispartof | CHEMISTRY & BIODIVERSITY | |
dc.subject | Artificial lipid bilayers | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Biosensors | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | DNA | |
dc.subject | Lipids | |
dc.subject | Oligonucleotides | |
dc.title | Specific DNA Duplex Formation at an Artificial Lipid Bilayer: towards a New DNA Biosensor Technology | |
dc.type | journal article | |
dc.identifier.doi | 10.1002/cbdv.201100298 | |
dc.identifier.isi | ISI:000300662300006 | |
dc.description.volume | 9 | |
dc.description.issue | 2 | |
dc.description.startpage | 272 | |
dc.description.endpage | 281 | |
dc.publisher.place | PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY | |
dcterms.isPartOf.abbreviation | Chem. Biodivers. | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | Institut für Chemie neuer Materialien | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | institute11 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | KoSe681 | - |
crisitem.author.netid | WaRi703 | - |
crisitem.author.netid | RoHe783 | - |
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geprüft am 01.06.2024