Stabilization of tandem dG-dA base pairs in DNA-hairpins: replacement of the canonical bases by 7-deaza-7-propynylpurines

DC FieldValueLanguage
dc.contributor.authorSeela, F
dc.contributor.authorBudow, S
dc.contributor.authorShaikh, KI
dc.contributor.authorJawalekar, AM
dc.date.accessioned2021-12-23T16:05:33Z-
dc.date.available2021-12-23T16:05:33Z-
dc.date.issued2005
dc.identifier.issn14770520
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7071-
dc.description.abstractThe stabilizing effect of 7-propynylated 7-deazapurine nucleosides on DNA-hairpins and DNA-duplexes containing d(GA) mismatches was investigated. The corresponding oligonucleotides were synthesized using solid-phase synthesis. For this purpose, the phosphoramidite of 7-deaza-7-propynyl-2'-deoxyadenosine (3c) was prepared. The incorporation of 3c instead of dA into the tandem d(GA) base pair of a DNA-hairpin alters the secondary structure, but has a positive effect on the duplex stability. A complete replacement of the canonical nucleosides of the tandem d(GA) base pair by 3c and 7-deaza-7-propynyl-2'-deoxyguanosine results in a significant base pair stabilization.
dc.language.isoen
dc.publisherROYAL SOC CHEMISTRY
dc.relation.ispartofORGANIC & BIOMOLECULAR CHEMISTRY
dc.subjectChemistry
dc.subjectChemistry, Organic
dc.subjectDUPLEX DNA
dc.subjectFRAGMENTS
dc.subjectG.A MISMATCHES
dc.subjectNMR
dc.subjectOLIGONUCLEOTIDES
dc.subjectSTABILITY
dc.subjectTHERMODYNAMICS
dc.titleStabilization of tandem dG-dA base pairs in DNA-hairpins: replacement of the canonical bases by 7-deaza-7-propynylpurines
dc.typejournal article
dc.identifier.doi10.1039/b510444k
dc.identifier.isiISI:000233362000007
dc.description.volume3
dc.description.issue23
dc.description.startpage4221
dc.description.endpage4226
dc.publisher.placeTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
dcterms.isPartOf.abbreviationOrg. Biomol. Chem.
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