Stabilization of tandem dG-dA base pairs in DNA-hairpins: replacement of the canonical bases by 7-deaza-7-propynylpurines
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Seela, F | |
dc.contributor.author | Budow, S | |
dc.contributor.author | Shaikh, KI | |
dc.contributor.author | Jawalekar, AM | |
dc.date.accessioned | 2021-12-23T16:05:33Z | - |
dc.date.available | 2021-12-23T16:05:33Z | - |
dc.date.issued | 2005 | |
dc.identifier.issn | 14770520 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/7071 | - |
dc.description.abstract | The stabilizing effect of 7-propynylated 7-deazapurine nucleosides on DNA-hairpins and DNA-duplexes containing d(GA) mismatches was investigated. The corresponding oligonucleotides were synthesized using solid-phase synthesis. For this purpose, the phosphoramidite of 7-deaza-7-propynyl-2'-deoxyadenosine (3c) was prepared. The incorporation of 3c instead of dA into the tandem d(GA) base pair of a DNA-hairpin alters the secondary structure, but has a positive effect on the duplex stability. A complete replacement of the canonical nucleosides of the tandem d(GA) base pair by 3c and 7-deaza-7-propynyl-2'-deoxyguanosine results in a significant base pair stabilization. | |
dc.language.iso | en | |
dc.publisher | ROYAL SOC CHEMISTRY | |
dc.relation.ispartof | ORGANIC & BIOMOLECULAR CHEMISTRY | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Organic | |
dc.subject | DUPLEX DNA | |
dc.subject | FRAGMENTS | |
dc.subject | G.A MISMATCHES | |
dc.subject | NMR | |
dc.subject | OLIGONUCLEOTIDES | |
dc.subject | STABILITY | |
dc.subject | THERMODYNAMICS | |
dc.title | Stabilization of tandem dG-dA base pairs in DNA-hairpins: replacement of the canonical bases by 7-deaza-7-propynylpurines | |
dc.type | journal article | |
dc.identifier.doi | 10.1039/b510444k | |
dc.identifier.isi | ISI:000233362000007 | |
dc.description.volume | 3 | |
dc.description.issue | 23 | |
dc.description.startpage | 4221 | |
dc.description.endpage | 4226 | |
dc.publisher.place | THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND | |
dcterms.isPartOf.abbreviation | Org. Biomol. Chem. |
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geprüft am 01.06.2024