Site-Directed Spin Labeling of DNA Reveals Mismatch-Induced Nanometer Distance Changes between Flanking Nucleotides
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Wunnicke, Dorith | |
dc.contributor.author | Ding, Ping | |
dc.contributor.author | Seela, Frank | |
dc.contributor.author | Steinhoff, Heinz-Juergen | |
dc.date.accessioned | 2021-12-23T16:05:37Z | - |
dc.date.available | 2021-12-23T16:05:37Z | - |
dc.date.issued | 2012 | |
dc.identifier.issn | 15206106 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/7100 | - |
dc.description.abstract | Multiple forms of DNA damages such as base modifications, double-strand breaks, and mispairings are related to inheritable diseases, cancer, and aging. Here, the structural changes of duplex DNA upon incorporation of mismatched base pairs are examined by EPR spectroscopy. Two ethynyl-7-deaza-2'-deoxyadenosine residues separated by two nucleotides were incorporated in DNA and functionalized with 4-azido-2,2,6,6-tetramethyl-piperidine-1-oxyl (4-azido TEMPO) by the click reaction. Mismatches such as dT.dT or dA.dA mispairs were positioned between these two spin labels in DNA duplexes. Pulse EPR experiments reveal that the mismatch-induced local conformational changes are transmitted to the flanking nucleotides and that the impact of this mismatch depends on the nearest neighbor environment. | |
dc.description.sponsorship | VolkswagenstiftungVolkswagen [I/79 950-952]; ChemBiotech, Munster, Germany; Financial support by the Volkswagenstiftung (I/79 950-952) (to D.W.) and by the ChemBiotech, Munster, Germany (to P.D.) is gratefully acknowledged. | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | JOURNAL OF PHYSICAL CHEMISTRY B | |
dc.subject | CENTER-DOT-T | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Physical | |
dc.subject | DAMAGE | |
dc.subject | DEER | |
dc.subject | DUPLEX DNA | |
dc.subject | EPR SPECTROSCOPY | |
dc.subject | NMR | |
dc.subject | NUCLEOSIDE | |
dc.subject | ORIENTATION | |
dc.subject | REPAIR | |
dc.subject | THERMODYNAMICS | |
dc.title | Site-Directed Spin Labeling of DNA Reveals Mismatch-Induced Nanometer Distance Changes between Flanking Nucleotides | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/jp212421c | |
dc.identifier.isi | ISI:000302591500002 | |
dc.description.volume | 116 | |
dc.description.issue | 14 | |
dc.description.startpage | 4118 | |
dc.description.endpage | 4123 | |
dc.contributor.orcid | 0000-0002-5888-0157 | |
dc.contributor.orcid | 0000-0002-5016-4041 | |
dc.contributor.researcherid | H-3791-2014 | |
dc.identifier.eissn | 15205207 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | J. Phys. Chem. B | |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | StHe633 | - |
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geprüft am 06.06.2024