Anomeric 2'-Deoxycytidines and Silver Ions: Hybrid Base Pairs with Greatly Enhanced Stability and Efficient DNA Mismatch Detection with alpha-dC

Autor(en): Guo, Xiurong
Seela, Frank
Stichwörter: BINDING; C-AG-C; Chemistry; Chemistry, Multidisciplinary; CRYSTAL-STRUCTURE; CYTOSINE; DNA; DUPLEX; metallo-base pairs; mismatches; NUCLEOBASE; NUCLEOSIDES; NUCLEOTIDES; silver; SPECTROSCOPY; STABILIZATION
Erscheinungsdatum: 2017
Herausgeber: WILEY-V C H VERLAG GMBH
Journal: CHEMISTRY-A EUROPEAN JOURNAL
Volumen: 23
Ausgabe: 49
Startseite: 11776
Seitenende: 11779
Zusammenfassung: 
alpha-D-Nucleosides are rare in nature but can develop fascinating properties when incorporated into DNA. This work reports on the first silver-mediated base pair constructed from two anomeric nucleosides: alpha-dC and beta-dC. The hybrid base pair was integrated into the DNA and DNA/RNA double helix. A 12-mer duplex with alpha-dC and beta-dC pair exhibits a higher thermal stability (T-m=43 degrees C) than that incorporating the beta-dC-Ag+ beta-dC homo pair (T-m=34 degrees C). Furthermore, alpha-dC shows excellent mismatch discrimination for DNA single nucleotide polymorphism (SNP). All four SNPs were identified on the basis of large T-m value differences measured in the presence of silver ions. High resolution melting was not required.
ISSN: 09476539
DOI: 10.1002/chem.201703017

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