The sensor kinase KdpD of Escherichia coli senses external K

DC FieldValueLanguage
dc.contributor.authorLaermann, Vera
dc.contributor.authorCudic, Emina
dc.contributor.authorKipschull, Kerstin
dc.contributor.authorZimmann, Petra
dc.contributor.authorAltendorf, Karlheinz
dc.date.accessioned2021-12-23T16:05:45Z-
dc.date.available2021-12-23T16:05:45Z-
dc.date.issued2013
dc.identifier.issn0950382X
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7183-
dc.description.abstractThe Kdp system of Escherichia coli is composed of the high-affinity K+ transporter KdpFABC and the two regulatory proteins KdpD (sensor kinase) and KdpE (response regulator), which constitute a typical two-component system. The kdpFABC operon is induced under K+-limiting conditions and, to a lesser extent, under high osmolality in the medium. In search for the stimulus sensed by KdpD, we studied the inhibitory effect of extracellular K+ on the Kdp system at pH6.0, which is masked by unspecific K+ transport at higher pH values. Based on KdpD derivatives carrying single aspartate replacements in the periplasmic loops which are part of the input domain, we concluded that the inhibition of the Kdp system at extracellular K+ concentrations above 5mM is mediated via KdpD/KdpE and not due to inhibition of the K+-transporting KdpFABC complex. Furthermore, time-course analyses of kdpFABC expression revealed that a decline in the extracellular K+ concentration efficiently stimulates KdpD/KdpE-mediated signal transduction. In this report we provide evidence that the extracellular K+ concentration serves as one of the stimuli sensed by KdpD.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG); Friedel & Gisela Bohnenkamp-Stiftung; Ministry for Research and Culture of the State of Lower Saxony; This work was financially supported by grants from the Deutsche Forschungsgemeinschaft, the Friedel & Gisela Bohnenkamp-Stiftung, and the Ministry for Research and Culture of the State of Lower Saxony. We thank Ryan Kitko for the gift of plasmid pMMB1311, Wolf Epstein for providing mutant strains of E. coli and Gabriele Deckers-Hebestreit for critically reading the manuscript.
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofMOLECULAR MICROBIOLOGY
dc.subjectATPASE
dc.subjectBiochemistry & Molecular Biology
dc.subjectCATION-TRANSPORT
dc.subjectDOMAIN
dc.subjectEXTENSION
dc.subjectMicrobiology
dc.subjectOPERON EXPRESSION
dc.subjectPH
dc.subjectPOTASSIUM-TRANSPORT
dc.subjectPROTEIN
dc.subjectSYSTEM
dc.subjectTURGOR
dc.titleThe sensor kinase KdpD of Escherichia coli senses external K
dc.typejournal article
dc.identifier.doi10.1111/mmi.12251
dc.identifier.isiISI:000320174300013
dc.description.volume88
dc.description.issue6
dc.description.startpage1194
dc.description.endpage1204
dc.identifier.eissn13652958
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationMol. Microbiol.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidAlKa770-
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