Horizontal Bilayer for Electrical and Optical Recordings

Autor(en): Bartsch, Philipp
Walter, Claudius
Selenschik, Philipp
Honigmann, Alf
Wagner, Richard 
Stichwörter: CELL-MEMBRANE STRUCTURE; Chemistry; Chemistry, Physical; CORRELATION SPECTROSCOPY; horizontal bilayer; LATERAL DIFFUSION; lipid diffusion; LIPID MONOLAYERS; Materials Science; Materials Science, Multidisciplinary; membrane dynamics; Metallurgy & Metallurgical Engineering; MOBILITY; Physics; Physics, Applied; Physics, Condensed Matter; PROTEIN; RECONSTITUTION; RESOLUTION; simultaneous electrical-optical recordings; SINGLE-PARTICLE TRACKING; supported bilayer; VESICLES
Erscheinungsdatum: 2012
Herausgeber: MDPI
Journal: MATERIALS
Volumen: 5
Ausgabe: 12
Startseite: 2705
Seitenende: 2730
Zusammenfassung: 
Artificial bilayer containing reconstituted ion channels, transporters and pumps serve as a well-defined model system for electrophysiological investigations of membrane protein structure-function relationship. Appropriately constructed microchips containing horizontally oriented bilayers with easy solution access to both sides provide, in addition, the possibility to investigate these model bilayer membranes and the membrane proteins therein with high resolution fluorescence techniques up to the single-molecule level. Here, we describe a bilayer microchip system in which long-term stable horizontal free-standing and hydrogel-supported bilayers can be formed and demonstrate its prospects particularly for single-molecule fluorescence spectroscopy and high resolution fluorescence microscopy in probing the physicochemical properties like phase behavior of the bilayer-forming lipids, as well as in functional studies of membrane proteins.
DOI: 10.3390/ma5122705

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