Horizontal Bilayer for Electrical and Optical Recordings

DC FieldValueLanguage
dc.contributor.authorBartsch, Philipp
dc.contributor.authorWalter, Claudius
dc.contributor.authorSelenschik, Philipp
dc.contributor.authorHonigmann, Alf
dc.contributor.authorWagner, Richard
dc.date.accessioned2021-12-23T16:05:45Z-
dc.date.available2021-12-23T16:05:45Z-
dc.date.issued2012
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7184-
dc.description.abstractArtificial bilayer containing reconstituted ion channels, transporters and pumps serve as a well-defined model system for electrophysiological investigations of membrane protein structure-function relationship. Appropriately constructed microchips containing horizontally oriented bilayers with easy solution access to both sides provide, in addition, the possibility to investigate these model bilayer membranes and the membrane proteins therein with high resolution fluorescence techniques up to the single-molecule level. Here, we describe a bilayer microchip system in which long-term stable horizontal free-standing and hydrogel-supported bilayers can be formed and demonstrate its prospects particularly for single-molecule fluorescence spectroscopy and high resolution fluorescence microscopy in probing the physicochemical properties like phase behavior of the bilayer-forming lipids, as well as in functional studies of membrane proteins.
dc.description.sponsorshipDFGGerman Research Foundation (DFG)European Commission [WA681/2-1, FOR967]; Ionovation GmbH Osnabrueck; We thank Birgit Hemmis (University of Osnabrueck) for technical help and Anke Harsman for critical discussion of the manuscript. Funding for this work was provided by DFG WA681/2-1 (to R.W.), DFG FOR967 (to R.W.), and Ionovation GmbH Osnabrueck.
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofMATERIALS
dc.subjectCELL-MEMBRANE STRUCTURE
dc.subjectChemistry
dc.subjectChemistry, Physical
dc.subjectCORRELATION SPECTROSCOPY
dc.subjecthorizontal bilayer
dc.subjectLATERAL DIFFUSION
dc.subjectlipid diffusion
dc.subjectLIPID MONOLAYERS
dc.subjectMaterials Science
dc.subjectMaterials Science, Multidisciplinary
dc.subjectmembrane dynamics
dc.subjectMetallurgy & Metallurgical Engineering
dc.subjectMOBILITY
dc.subjectPhysics
dc.subjectPhysics, Applied
dc.subjectPhysics, Condensed Matter
dc.subjectPROTEIN
dc.subjectRECONSTITUTION
dc.subjectRESOLUTION
dc.subjectsimultaneous electrical-optical recordings
dc.subjectSINGLE-PARTICLE TRACKING
dc.subjectsupported bilayer
dc.subjectVESICLES
dc.titleHorizontal Bilayer for Electrical and Optical Recordings
dc.typejournal article
dc.identifier.doi10.3390/ma5122705
dc.identifier.isiISI:000312608500015
dc.description.volume5
dc.description.issue12
dc.description.startpage2705
dc.description.endpage2730
dc.contributor.orcid0000-0003-0475-3790
dc.contributor.researcheridC-4832-2017
dc.identifier.eissn19961944
dc.publisher.placeST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
dcterms.isPartOf.abbreviationMaterials
dcterms.oaStatusGreen Submitted, gold, Green Published
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWaRi703-
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