DC Field | Value | Language |
dc.contributor.author | Limar, Sergej | |
dc.contributor.author | Koerner, Carolin | |
dc.contributor.author | Martinez-Montanes, Fernando | |
dc.contributor.author | Stancheva, Viktoriya G. | |
dc.contributor.author | Wolf, Verena N. | |
dc.contributor.author | Walter, Stefan | |
dc.contributor.author | Miller, Elizabeth A. | |
dc.contributor.author | Ejsing, Christer S. | |
dc.contributor.author | Galassi, Vanesa Viviana | |
dc.contributor.author | Froehlich, Florian | |
dc.date.accessioned | 2023-07-12T06:57:21Z | - |
dc.date.available | 2023-07-12T06:57:21Z | - |
dc.date.issued | 2023 | |
dc.identifier.issn | 0021-9525 | |
dc.identifier.uri | http://osnascholar.ub.uni-osnabrueck.de/handle/unios/71982 | - |
dc.description.abstract | Limar et al. show that the yeast protein Svf1 localizes to the cis Golgi apparatus via an N-terminal amphipathic helix. Svf1 directly binds ceramides and is necessary to maintain appropriate levels of complex sphingolipids, most likely by transporting ceramides between the ER and the Golgi apparatus. Ceramides are essential precursors of complex sphingolipids and act as potent signaling molecules. Ceramides are synthesized in the endoplasmic reticulum (ER) and receive their head-groups in the Golgi apparatus, yielding complex sphingolipids (SPs). Transport of ceramides between the ER and the Golgi is executed by the essential ceramide transport protein (CERT) in mammalian cells. However, yeast cells lack a CERT homolog, and the mechanism of ER to Golgi ceramide transport remains largely elusive. Here, we identified a role for yeast Svf1 in ceramide transport between the ER and the Golgi. Svf1 is dynamically targeted to membranes via an N-terminal amphipathic helix (AH). Svf1 binds ceramide via a hydrophobic binding pocket that is located in between two lipocalin domains. We showed that Svf1 membrane-targeting is important to maintain flux of ceramides into complex SPs. Together, our results show that Svf1 is a ceramide binding protein that contributes to sphingolipid metabolism at Golgi compartments. | |
dc.description.sponsorship | VILLUM Foundation [VKR023439]; VILLUM Center for Bioanalytical Sciences [VKR023179]; Lundbeckfonden [R54-A5858]; UK Medical Research Council [MRC_UP_12-1/10]; DFG [FR 3647/3-1, FR 3647/4-1, SFB944]; We thank members of the Froehlich and Ungermann labs for critical comments on the manuscript, and Alejandro Melero Carrillo for advice on COPII budding for lipidomics.This research was supported by the VILLUM Foundation (VKR023439 to C.S. Ejsing), the VILLUM Center for Bioanalytical Sciences (VKR023179 to C.S. Ejsing), the Lundbeckfonden (R54-A5858, C.S. Ejsing) and the UK Medical Research Council (MRC_UP_12-1/10 to E.A. Miller). Sergej Limar is a member of the UOS funded graduate school ``EvoCell.'' Florian Froehlich is supported by the DFG grants FR 3647/3-1, FR 3647/4-1, and SFB944. | |
dc.language.iso | en | |
dc.publisher | ROCKEFELLER UNIV PRESS | |
dc.relation.ispartof | JOURNAL OF CELL BIOLOGY | |
dc.subject | Cell Biology | |
dc.subject | DATABASE | |
dc.subject | GLUCOSYLCERAMIDE | |
dc.subject | HOMEOSTASIS | |
dc.subject | HYDROLYSIS | |
dc.subject | IDENTIFICATION | |
dc.subject | INHIBITION | |
dc.subject | PHOSPHATIDYLSERINE TRANSPORT | |
dc.subject | PROTEIN | |
dc.subject | SPECTROMETRY-BASED PROTEOMICS | |
dc.subject | TRAFFICKING | |
dc.title | Yeast Svf1 binds ceramides and contributes to sphingolipid metabolism at the ER cis-Golgi interface | |
dc.type | journal article | |
dc.identifier.doi | 10.1083/jcb.202109162 | |
dc.identifier.isi | ISI:000978306300001 | |
dc.description.volume | 222 | |
dc.description.issue | 5 | |
dc.contributor.orcid | http://orcid.org/0000-0001-8307-2189 | |
dc.contributor.orcid | http://orcid.org/0000-0002-6642-7555 | |
dc.contributor.orcid | http://orcid.org/0000-0002-8797-3184 | |
dc.contributor.orcid | http://orcid.org/0000-0002-1033-8369 | |
dc.contributor.orcid | http://orcid.org/0000-0003-4963-0276 | |
dc.contributor.researcherid | R-5922-2016 | |
dc.contributor.researcherid | G-3883-2012 | |
dc.identifier.eissn | 1540-8140 | |
dc.publisher.place | 950 THIRD AVE, 2ND FLR, NEW YORK, NY 10022 USA | |
dcterms.isPartOf.abbreviation | J. Cell Biol. | |
local.import.remains | affiliations : University Osnabruck; University of Southern Denmark; MRC Laboratory Molecular Biology; European Molecular Biology Laboratory (EMBL); University Nacional Cuyo Mendoza; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); University Nacional Cuyo Mendoza | |
local.import.remains | web-of-science-index : Science Citation Index Expanded (SCI-EXPANDED) | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0001-8307-2189 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | FrFl166 | - |