TEV protease cleavage in generation of artificial substrate proteins bearing neo-N-termini
DC Element | Wert | Sprache |
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dc.contributor.author | Winckler, Lioba Inken | |
dc.contributor.author | Dissmeyer, Nico | |
dc.date.accessioned | 2023-07-12T06:59:26Z | - |
dc.date.available | 2023-07-12T06:59:26Z | - |
dc.date.issued | 2023 | |
dc.identifier.issn | 0076-6879 | |
dc.identifier.uri | http://osnascholar.ub.uni-osnabrueck.de/handle/unios/72073 | - |
dc.description | Cited by: 1 | |
dc.description.abstract | The tobacco etch virus (TEV) protease is widely used in in vitro and in vivo approaches for the removal of affinity tags from fusion proteins or the generation of proteins with a desired N-terminal amino acid. Processing of fusion proteins by the TEV protease can either be achieved by encoding the TEV protease and its recognition site on one construct (self-cleavage) or on two different constructs (co-expression). Here, we compare the efficiency of the self-splitting approach to the co-expression approach. © 2023 Elsevier Inc. | |
dc.language.iso | en | |
dc.publisher | Academic Press Inc. | |
dc.relation.ispartof | Methods in Enzymology | |
dc.subject | N-degron pathway | |
dc.subject | Proteases | |
dc.subject | Protein degradation | |
dc.subject | TEV | |
dc.title | TEV protease cleavage in generation of artificial substrate proteins bearing neo-N-termini | |
dc.type | book part | |
dc.identifier.doi | 10.1016/bs.mie.2023.02.015 | |
dc.identifier.scopus | 2-s2.0-85151633999 | |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151633999&doi=10.1016%2fbs.mie.2023.02.015&partnerID=40&md5=b5bc9f862ffa1c77d646681df9ab96bb | |
dcterms.isPartOf.abbreviation | Methods Enzymol. | |
local.import.remains | affiliations : Department of Plant Physiology and Protein Metabolism Laboratory, University of Osnabruck, Osnabruck, Germany; CellNanOs—Center of Cellular Nanoanalytics, University of Osnabruck, Osnabruck, Germany; Faculty of Biology, University of Osnabruck, Osnabruck, Germany | |
local.import.remains | correspondence_address : N. Dissmeyer; Department of Plant Physiology and Protein Metabolism Laboratory, University of Osnabruck, Osnabruck, Germany; email: nico.dissmeyer@uni-osnabrueck.de | |
local.import.remains | publication_stage : Article in press | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-4156-3761 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | DiNi018 | - |
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geprüft am 08.06.2024