Dual-Color Real-Time Chemosensing of a Compartmentalized Reaction Network Involving Enzyme-Induced Membrane Permeation of Peptides
DC Element | Wert | Sprache |
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dc.contributor.author | Jiang, Ruixue | |
dc.contributor.author | Nilam, Mohamed | |
dc.contributor.author | Hennig, Andreas | |
dc.contributor.author | Nau, Werner M. | |
dc.date.accessioned | 2024-01-04T10:28:52Z | - |
dc.date.available | 2024-01-04T10:28:52Z | - |
dc.date.issued | 2023 | |
dc.identifier.issn | 0935-9648 | |
dc.identifier.uri | http://osnascholar.ub.uni-osnabrueck.de/handle/unios/72915 | - |
dc.description | Cited by: 0; All Open Access, Bronze Open Access | |
dc.description.abstract | The design of synthetic systems with interrelated reaction sequences that model incipient biological complexity is limited by physicochemical tools that allow the direct monitoring of the individual processes in real-time. To mimic a simple digestion-resorption sequence, the authors have designed compartmentalized liposomal systems that incorporate extra- and intravesicular chemosensing ensembles. The extravesicular reporter pair consists of cucurbit[7]uril and methylene blue to monitor the enzymatic cleavage of short enkephalin-related peptides by thermolysin through a switch-off fluorescence response (“digestion”). Because the substrate is membrane-impermeable, but the dipeptide product is permeable, uptake of the latter into the pre-formed liposomes occurs as a follow-up process. The intravesicular chemosensing ensemble consists of i) cucurbit[8]uril, 2-anilinonaphthalene-6-sulfonic acid, and methyl viologen or ii) cucurbit[7]uril and berberine to monitor the uptake (“resorption”) of the enzymatic products through the liposomal membranes by i) a switch-on or ii) a switch-off fluorescence response. The dyes are designed to allow selective optical excitation and read-out of the extra- and intravesicular dyes, which allow for dual-color chemosensing and, therefore, kinetic discrimination of the two sequential reactions. © 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH. | |
dc.language.iso | en | |
dc.publisher | John Wiley and Sons Inc | |
dc.relation.ispartof | Advanced Materials | |
dc.subject | Aromatic compounds | |
dc.subject | assays | |
dc.subject | Biological complexity | |
dc.subject | Chemosensing | |
dc.subject | Cucurbit[7]uril | |
dc.subject | cucurbiturils | |
dc.subject | Dual color | |
dc.subject | dyes | |
dc.subject | fluorescence | |
dc.subject | kinetics | |
dc.subject | Liposomes | |
dc.subject | Membrane permeation | |
dc.subject | Peptides | |
dc.subject | Reaction kinetics | |
dc.subject | Reaction network | |
dc.subject | Reaction sequences | |
dc.subject | Real time systems | |
dc.subject | Real- time | |
dc.subject | supramolecular chemistry | |
dc.subject | Synthetic systems | |
dc.title | Dual-Color Real-Time Chemosensing of a Compartmentalized Reaction Network Involving Enzyme-Induced Membrane Permeation of Peptides | |
dc.type | journal article | |
dc.identifier.doi | 10.1002/adma.202306922 | |
dc.identifier.scopus | 2-s2.0-85177606121 | |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85177606121&doi=10.1002%2fadma.202306922&partnerID=40&md5=7af29fb9354a2d1af8c55f847a666222 | |
dcterms.isPartOf.abbreviation | Adv Mater | |
local.import.remains | affiliations : School of Science, Constructor University, Campus Ring 1, Bremen, 28759, Germany; Center for Cellular Nanoanalytics (CellNanOs), Department of Biology and Chemistry, Universität Osnabrück, Barbarastraße 7, Osnabrück, 49069, Germany | |
local.import.remains | correspondence_address : W.M. Nau; School of Science, Constructor University, Bremen, Campus Ring 1, 28759, Germany; email: wnau@constructor.university; A. Hennig; Center for Cellular Nanoanalytics (CellNanOs), Department of Biology and Chemistry, Universität Osnabrück, Osnabrück, Barbarastraße 7, 49069, Germany; email: andreas.hennig@uni-osnabrueck.de | |
local.import.remains | publication_stage : Article in press | |
crisitem.author.dept | Institut für Chemie neuer Materialien | - |
crisitem.author.deptid | institute11 | - |
crisitem.author.orcid | 0000-0003-0444-5923 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | HeAn175 | - |
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geprüft am 07.06.2024