Next Generation of Nonlinear Laser Microscopy Based on High Energy Fs-Pulses: Widefield Mode up to 2.2 microns

Abstract

State of the art nonlinear microscopes are based on non-regeneratively amplified fs-laser having a typical repetition rate of ν ≈ 80 MHz and a maximum energy per pulse of Ep ≈ 100 nJ. In this work the use of a different type of laser technology, the one of regeneratively amplified laser, is explored. These systems can deliver pulse energies up to Ep ≈ 1 mJ with a repetition rate up to ≈ 1 MHz. This choice was already proven to be beneficial to improve the imaging depth [1] but it brings other unexplored advantages, which are the heart of the recently conceived Tunable hIGh EneRgy (TIGER) microscope [2]. © 2023 IEEE.