Affinity capturing for targeting proteins into micro and nanostructures

Autor(en): You, Changjiang 
Bhagawati, Maniraj
Brecht, Andreas
Piehler, Jacob 
Stichwörter: Biochemical Research Methods; Biochemistry & Molecular Biology; Biotinylation; Chemistry; Chemistry, Analytical; DIP-PEN NANOLITHOGRAPHY; FUNCTIONAL IMMOBILIZATION; HISTIDINE-TAGGED PROTEINS; Microstructure; MOLECULAR PRINTBOARDS; NANOIMPRINT LITHOGRAPHY; Nanostructure; Nitrilotriacetic acid; Patterning; PHOSPHOPANTETHEINYL TRANSFERASE; POLY(ETHYLENE GLYCOL); Protein immobilization; SCANNING PROBE LITHOGRAPHY; SELF-ASSEMBLED MONOLAYERS; SURFACE-PLASMON RESONANCE
Erscheinungsdatum: 2009
Herausgeber: SPRINGER HEIDELBERG
Journal: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volumen: 393
Ausgabe: 6-7
Startseite: 1563
Seitenende: 1570
Zusammenfassung: 
Protein immobilization into micro and nano-scaled patterns opens exciting possibilities in fundamental and applied research. Developing efficient capturing techniques while preserving the structural and functional integrity of the proteins on surfaces is a key challenge for surface scientists. In this paper, current techniques for site-specific protein immobilization into engineered surface architectures are reviewed. Fundamental principles for functional protein immobilization on solid supports are discussed and popular affinity-based recognition pairs and their application for capturing proteins into nano and microstructures are presented.
ISSN: 16182642
DOI: 10.1007/s00216-008-2595-6

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