Affinity capturing for targeting proteins into micro and nanostructures
Autor(en): | You, Changjiang Bhagawati, Maniraj Brecht, Andreas Piehler, Jacob |
Stichwörter: | Biochemical Research Methods; Biochemistry & Molecular Biology; Biotinylation; Chemistry; Chemistry, Analytical; DIP-PEN NANOLITHOGRAPHY; FUNCTIONAL IMMOBILIZATION; HISTIDINE-TAGGED PROTEINS; Microstructure; MOLECULAR PRINTBOARDS; NANOIMPRINT LITHOGRAPHY; Nanostructure; Nitrilotriacetic acid; Patterning; PHOSPHOPANTETHEINYL TRANSFERASE; POLY(ETHYLENE GLYCOL); Protein immobilization; SCANNING PROBE LITHOGRAPHY; SELF-ASSEMBLED MONOLAYERS; SURFACE-PLASMON RESONANCE | Erscheinungsdatum: | 2009 | Herausgeber: | SPRINGER HEIDELBERG | Journal: | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | Volumen: | 393 | Ausgabe: | 6-7 | Startseite: | 1563 | Seitenende: | 1570 | Zusammenfassung: | Protein immobilization into micro and nano-scaled patterns opens exciting possibilities in fundamental and applied research. Developing efficient capturing techniques while preserving the structural and functional integrity of the proteins on surfaces is a key challenge for surface scientists. In this paper, current techniques for site-specific protein immobilization into engineered surface architectures are reviewed. Fundamental principles for functional protein immobilization on solid supports are discussed and popular affinity-based recognition pairs and their application for capturing proteins into nano and microstructures are presented. |
ISSN: | 16182642 | DOI: | 10.1007/s00216-008-2595-6 |
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geprüft am 29.03.2024