Phylogenomic analysis identifies a sodium-translocating decarboxylating oxidoreductase in thermotogae

DC FieldValueLanguage
dc.contributor.authorKlimchuk, O. I.
dc.contributor.authorDibrova, D. V.
dc.contributor.authorMulkidjanian, A. Y.
dc.date.accessioned2021-12-23T16:06:47Z-
dc.date.available2021-12-23T16:06:47Z-
dc.date.issued2016
dc.identifier.issn00062979
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7548-
dc.description.abstractBacterial sodium-dependent decarboxylases were the first enzymes exemplifying sodium-dependent bioenergetics. These enzyme complexes couple decarboxylation of organic acids with the export of sodium ions via a special membrane subunit. In 711 representative prokaryotic genomes, we have analyzed genomic neighborhoods of the genes that code the membrane subunit of sodium decarboxylases. In representatives of Thermotogae, the operons with the gene of this subunit lack the genes of subunits that perform non-oxidative decarboxylation. Instead, these operons contain the genes of alphaand delta-subunits of decarboxylating oxidoreductases of alpha-ketoacids. The genes of betaand gamma-subunits of the decarboxylating oxidoreductases were found within the genomes of respective Thermotogae species as separate, twogene operons. We suggest that the described two operons code together for sodium-translocating decarboxylating oxidoreductases capable of coupling oxidative decarboxylation of alpha-ketoacids with the export of sodium ions, which is a novel type of bioenergetic coupling.
dc.description.sponsorshipRussian Science FoundationRussian Science Foundation (RSF) [14-50-00029, 14-14-00592]; This work was supported by the Russian Science Foundation (projects No. 14-50-00029, O. I. Klimchuk, phylogenomic analysis of redox-dependent decarboxylases; No. 14-14-00592, D. V. Dibrova and A. Y. Mulkidjanian, phylogenetic analysis of sodium-translocating energy converting membrane proteins).
dc.language.isoen
dc.publisherMAIK NAUKA/INTERPERIODICA/SPRINGER
dc.relation.ispartofBIOCHEMISTRY-MOSCOW
dc.subjectanaerobic bacteria
dc.subjectBiochemistry & Molecular Biology
dc.subjectcomparative genomics
dc.subjectCOMPLEX
dc.subjectCRYSTAL-STRUCTURE
dc.subjectELECTRON-TRANSPORT
dc.subjectferredoxin
dc.subjectION PUMP
dc.subjectMALONATE DECARBOXYLASE
dc.subjectMALONOMONAS-RUBRA
dc.subjectmembrane bioenergetics
dc.subjectmolecular evolution
dc.subjectNA+ PUMP
dc.subjectphylogenetic analysis
dc.subjectPYRUVATE-FERREDOXIN OXIDOREDUCTASE
dc.subjectsodium transport
dc.subjectTRANSPORT-SYSTEM
dc.subjectVACUOLAR-TYPE
dc.titlePhylogenomic analysis identifies a sodium-translocating decarboxylating oxidoreductase in thermotogae
dc.typejournal article
dc.identifier.doi10.1134/S0006297916050059
dc.identifier.isiISI:000376411600005
dc.description.volume81
dc.description.issue5
dc.description.startpage481
dc.description.endpage490
dc.contributor.orcid0000-0001-5844-3064
dc.contributor.orcid0000-0002-6954-6437
dc.contributor.researcheridJ-8086-2013
dc.contributor.researcheridAAH-3608-2021
dc.contributor.researcheridC-6003-2014
dc.identifier.eissn16083040
dc.publisher.place233 SPRING ST, NEW YORK, NY 10013-1578 USA
dcterms.isPartOf.abbreviationBiochem.-Moscow
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