1',5'-anhydrohexitol oligonucleotides: Synthesis, base pairing and recognition by regular oligodeoxyribonucleotides and oligoribonucleotides

Autor(en): Hendrix, C
Rosemeyer, H 
Verheggen, I
Seela, F
VanAerschot, A
Herdewijn, P
Stichwörter: 1,5-ANHYDROHEXITOL NUCLEOSIDES; ANALOGS; antisense systems; BINDING; BUILDING-BLOCKS; Chemistry; Chemistry, Multidisciplinary; DNA recognition; ENZYMATIC STABILITY; HEXOSE-NUCLEIC-ACIDS; HOMO-DNA; HYBRIDIZATION; nucleic acids; OLIGODEOXYNUCLEOTIDES; oligonucleotides; RNA; SOLID-PHASE SYNTHESIS
Erscheinungsdatum: 1997
Herausgeber: WILEY-V C H VERLAG GMBH
Journal: CHEMISTRY-A EUROPEAN JOURNAL
Volumen: 3
Ausgabe: 1
Startseite: 110
Seitenende: 120
Zusammenfassung: 
Oligonucleotides constructed of 1',5'-anhydrohexitol nucleoside building blocks (hexitol nucleic acids, HNA) are completely stable towards 3'-exonuclease and form very stable self-complementary duplexes as well as sequence-selective stable duplexes with the natural DNA and RNA, Triple-helix formation has also been observed, These hybridisation characteristics are highly dependent on the base sequence and the experimental conditions, When using a phosphate buffer containing 0.1M NaCl, a homopurine HNA dodecamer gives a Delta T-m of 1.3 degrees C/ base pair with DNA as complement and a Delta T-m of 3.0 degrees C/base pair with RNA as complement, These oligomers may therefore be of considerable interest as antisense constructs.
ISSN: 09476539
DOI: 10.1002/chem.19970030118

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