TRKH AND ITS HOMOLOG, TRKG, DETERMINE THE SPECIFICITY AND KINETICS OF CATION-TRANSPORT BY THE TRK SYSTEM OF ESCHERICHIA-COLI

DC FieldValueLanguage
dc.contributor.authorSCHLOSSER, A
dc.contributor.authorMELDORF, M
dc.contributor.authorSTUMPE, S
dc.contributor.authorBAKKER, EP
dc.contributor.authorEPSTEIN, W
dc.date.accessioned2021-12-23T16:06:53Z-
dc.date.available2021-12-23T16:06:53Z-
dc.date.issued1995
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7601-
dc.description.abstractThe corrected sequence of the trkH gene of Escherichia coli predicts that the TrkH protein is a hydrophobic membrane protein of 483 amino acid residues, of which 41% are identical to those of the homologous and functionally analogous TrkG protein. These two proteins form the transmembrane component of the Trk system for the uptake of K+. Each protein alone is sufficient for high-level Trk activity. When Trk is assembled with the TrkG protein, Rb+ and K+ are transported with a K-m near or below 1 mM; however, the V-max for Rb+ is only about 7% of that for K+. When Trk is formed with TrkH, the affinities for both for K+ and Rb+ are somewhat lower, and the V-max for Rb+ is only 1% of that for K+ transport. The kinetics of transport in strains with wild-type alleles at trkG and at trkH suggest that both products participate in transport.
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectBACTERIOPHAGE-T7 RNA-POLYMERASE
dc.subjectEXPRESSION
dc.subjectGENETIC-ANALYSIS
dc.subjectMEMBRANE-PROTEIN
dc.subjectMicrobiology
dc.subjectNUCLEOTIDE-SEQUENCE
dc.subjectPOTASSIUM UPTAKE
dc.titleTRKH AND ITS HOMOLOG, TRKG, DETERMINE THE SPECIFICITY AND KINETICS OF CATION-TRANSPORT BY THE TRK SYSTEM OF ESCHERICHIA-COLI
dc.typenote
dc.identifier.doi10.1128/jb.177.7.1908-1910.1995
dc.identifier.isiISI:A1995QP81000040
dc.description.volume177
dc.description.issue7
dc.description.startpage1908
dc.description.endpage1910
dc.publisher.place1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171
dcterms.isPartOf.abbreviationJ. Bacteriol.
dcterms.oaStatusBronze, Green Published
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