Dendrite tapering actuates a self-organizing signaling circuit for stochastic filopodia initiation in neurons
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Mancinelli, Gloria | |
dc.contributor.author | Lamparter, Lucas | |
dc.contributor.author | Nosov, Georgii | |
dc.contributor.author | Saha, Tanumoy | |
dc.contributor.author | Pawluchin, Anna | |
dc.contributor.author | Kurre, Rainer | |
dc.contributor.author | Rasch, Christiane | |
dc.contributor.author | Ebrahimkutty, Mirsana | |
dc.contributor.author | Klingauf, Juergen | |
dc.contributor.author | Galic, Milos | |
dc.date.accessioned | 2021-12-23T16:06:55Z | - |
dc.date.available | 2021-12-23T16:06:55Z | - |
dc.date.issued | 2021 | |
dc.identifier.issn | 00278424 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/7616 | - |
dc.description.abstract | How signaling units spontaneously arise from a noisy cellular background is not well understood. Here, we show that stochastic membrane deformations can nucleate exploratory dendritic filopodia, dynamic actin-rich structures used by neurons to sample its surroundings for compatible transcellular contacts. A theoretical analysis demonstrates that corecruitment of positive and negative curvature-sensitive proteins to deformed membranes minimizes the free energy of the system, allowing the formation of longlived curved membrane sections from stochastic membrane fluctuations. Quantitative experiments show that once recruited, curvature-sensitive proteins form a signaling circuit composed of interlinked positive and negative actin-regulatory feedback loops. As the positive but not the negative feedback loop can sense the dendrite diameter, this self-organizing circuit determines filopodia initiation frequency along tapering dendrites. Together, our findings identify a receptor-independent signaling circuit that employs random membrane deformations to simultaneously elicit and limit formation of exploratory filopodia to distal dendritic sites of developing neurons. | |
dc.description.sponsorship | German Research Foundation (DFG)German Research Foundation (DFG) [CRC1348/A06, CRC 944/P22, GA 2268/4-1]; Medical Faculty of the University of Munster [IZKF Ga3/016/21]; DFGGerman Research Foundation (DFG)European Commission [CRC1348/A02, CRC944/P5, PI 405/14-1]; We thank Lukas Funke, Anastasiia Sokolova, and Katrin Tkotz for technical support. We further thank members of the M.G., Matis, and J.K. laboratories for critical feedback and discussion. M.G. acknowledges funding from the German Research Foundation (DFG) (CRC1348/A06, CRC 944/P22, GA 2268/4-1) and the Medical Faculty of the University of M_unster (IZKF Ga3/016/21). J.K. acknowledges funding from the DFG (CRC1348/A02, CRC944/P5). The imaging facility Integrated Bioimaging Facility Osnabr_uck was funded by the DFG as a core facility (PI 405/14-1). | |
dc.language.iso | en | |
dc.publisher | NATL ACAD SCIENCES | |
dc.relation.ispartof | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | |
dc.subject | ACTIN DYNAMICS | |
dc.subject | ACTIVATION | |
dc.subject | BAR domain | |
dc.subject | CDC42 | |
dc.subject | filopodia | |
dc.subject | INHIBITION | |
dc.subject | IRSP53 | |
dc.subject | MECHANISMS | |
dc.subject | MEMBRANE | |
dc.subject | membrane curvature | |
dc.subject | MORPHOGENESIS | |
dc.subject | Multidisciplinary Sciences | |
dc.subject | neuron | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | self-organization | |
dc.subject | SPINES | |
dc.subject | SYNAPTOGENESIS | |
dc.title | Dendrite tapering actuates a self-organizing signaling circuit for stochastic filopodia initiation in neurons | |
dc.type | journal article | |
dc.identifier.doi | 10.1073/pnas.2106921118j1of9 | |
dc.identifier.isi | ISI:000715115300016 | |
dc.description.volume | 118 | |
dc.description.issue | 43 | |
dc.contributor.orcid | 0000-0002-4493-2542 | |
dc.publisher.place | 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA | |
dcterms.isPartOf.abbreviation | Proc. Natl. Acad. Sci. U. S. A. | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-6872-6567 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | KuRa617 | - |
Seitenaufrufe
2
Letzte Woche
0
0
Letzter Monat
0
0
geprüft am 23.05.2024