Formation and function of intracardiac valve cells in the Drosophila heart

DC FieldValueLanguage
dc.contributor.authorLammers, Kay
dc.contributor.authorAbeln, Bettina
dc.contributor.authorHuesken, Mirko
dc.contributor.authorLehmacher, Christine
dc.contributor.authorPsathaki, Olympia Ekaterini
dc.contributor.authorAlcorta, Esther
dc.contributor.authorMeyer, Heiko
dc.contributor.authorPaululat, Achim
dc.date.accessioned2021-12-23T16:07:05Z-
dc.date.available2021-12-23T16:07:05Z-
dc.date.issued2017
dc.identifier.issn00220949
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7700-
dc.description.abstractDrosophila harbours a simple tubular heart that ensures haemolymph circulation within the body. The heart is built by a few different cell types, including cardiomyocytes that define the luminal heart channel and ostia cells that constitute openings in the heart wall allowing haemolymph to enter the heart chamber. Regulation of flow directionality within a tube, such as blood flow in arteries or insect haemolymph within the heart lumen, requires a dedicated gate, valve or flap-like structure that prevents backflow of fluids. In the Drosophila heart, intracardiac valves provide this directionality of haemolymph streaming, with one valve being present in larvae and three valves in the adult fly. Each valve is built by two specialised cardiomyocytes that exhibit a unique histology. We found that the capacity to open and close the heart lumen relies on a unique myofibrillar setting as well as on the presence of large membranous vesicles. These vesicles are of endocytic origin and probably represent unique organelles of valve cells. Moreover, we characterised the working mode of the cells in real time. Valve cells exhibit a highly flexible shape and, during each heartbeat, oscillating shape changes result in closing and opening of the heart channel. Finally, we identified a set of novel valve cell markers useful for future in-depth analyses of cell differentiation in wild-type and mutant animals.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 944]; Deutscher Akademischer Austauschdienst (IPID program)Deutscher Akademischer Austausch Dienst (DAAD); State of Lower-SaxonyEuropean Commission [ZN2832]; State of Lower-Saxony (Lichtenberg-Stipendium); Ministerio de Economia y CompetitividadSpanish Government [SAF2013-48759-P]; Principado de Asturias [GRUPIN14-012]; This work was supported by grants from the Deutsche Forschungsgemeinschaft to A.P. (SFB 944), the Deutscher Akademischer Austauschdienst (IPID program) and from the State of Lower-Saxony (ZN2832). B.A. received a fellowship from the State of Lower-Saxony (Lichtenberg-Stipendium). E.A. was supported by grants from the Ministerio de Economia y Competitividad [SAF2013-48759-P] and the Principado de Asturias [GRUPIN14-012].
dc.language.isoen
dc.publisherCOMPANY BIOLOGISTS LTD
dc.relation.ispartofJOURNAL OF EXPERIMENTAL BIOLOGY
dc.subjectBiology
dc.subjectCardiac valves
dc.subjectCARDIAC-FUNCTION
dc.subjectCardiogenesis
dc.subjectDIFFERENTIATION
dc.subjectDORSAL VESSEL
dc.subjectDrosophila melanogaster
dc.subjectEndocytosis
dc.subjectEXPRESSION
dc.subjectGENE
dc.subjectHEMOLYMPH CIRCULATION
dc.subjectLife Sciences & Biomedicine - Other Topics
dc.subjectMECHANICS
dc.subjectMORPHOLOGY
dc.subjectMUTATIONS
dc.subjectTRANSCRIPTION FACTOR
dc.titleFormation and function of intracardiac valve cells in the Drosophila heart
dc.typejournal article
dc.identifier.doi10.1242/jeb.156265
dc.identifier.isiISI:000401483300017
dc.description.volume220
dc.description.issue10
dc.description.startpage1852
dc.description.endpage1863
dc.contributor.orcid0000-0002-8845-6859
dc.contributor.orcid0000-0002-3304-4523
dc.identifier.eissn14779145
dc.publisher.placeBIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND
dcterms.isPartOf.abbreviationJ. Exp. Biol.
dcterms.oaStatusGreen Published, hybrid
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-4035-6840-
crisitem.author.orcid0000-0002-8845-6859-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPsOl764-
crisitem.author.netidPaAc947-
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