Structure of the Vacuolar H+-ATPase Rotary Motor Reveals New Mechanistic Insights

DC FieldValueLanguage
dc.contributor.authorRawson, Shaun
dc.contributor.authorPhillips, Clair
dc.contributor.authorHuss, Markus
dc.contributor.authorTiburcy, Felix
dc.contributor.authorWieczorek, Helmut
dc.contributor.authorTrinick, John
dc.contributor.authorHarrison, Michael A.
dc.contributor.authorMuench, Stephen P.
dc.date.accessioned2021-12-23T16:07:16Z-
dc.date.available2021-12-23T16:07:16Z-
dc.date.issued2015
dc.identifier.issn09692126
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7791-
dc.description.abstractVacuolar H+-ATPases are multisubunit complexes that operate with rotary mechanics and are essential for membrane proton transport throughout eukaryotes. Here we report a similar to 1 nm resolution reconstruction of a V-ATPase in a different conformational state from that previously reported for a lower-resolution yeast model. The stator network of the V-ATPase (and by implication that of other rotary ATPases) does not change conformation in different catalytic states, and hence must be relatively rigid. We also demonstrate that a conserved bearing in the catalytic domain is electrostatic, contributing to the extraordinarily high efficiency of rotary ATPases. Analysis of the rotor axle/membrane pump interface suggests how rotary ATPases accommodate different c ring stoichiometries while maintaining high efficiency. The model provides evidence for a half channel in the proton pump, supporting theoretical models of ion translocation. Our refined model therefore provides new insights into the structure and mechanics of the V-ATPases.
dc.description.sponsorshipMRC career development grantUK Research & Innovation (UKRI)Medical Research Council UK (MRC) [G100567]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 944]; MRCUK Research & Innovation (UKRI)Medical Research Council UK (MRC) [G1000567] Funding Source: UKRI; Medical Research CouncilUK Research & Innovation (UKRI)Medical Research Council UK (MRC)European Commission [G1000567] Funding Source: researchfish; We thank the MRC Laboratory of Molecular Biology, Cambridge for access to the Titan-Krios microscope and Shaoxia Chen, Greg McMullan, and Christos Savva for assistance with data collection. We thank Martin Dransmann (Osnabruck) for his excellent technical assistance. This work was supported by an MRC career development grant to S.P.M. (G100567) and from the Deutsche Forschungsgemeinschaft (SFB 944) to H.W. S.R. is a Wellcome Trust supported PhD student (009752/Z/12/Z). Electron density map data have been deposited with the EMDB database (ID code EMD-2781 at www.ebi.ac.uk/msd-srv/docs/emdb/).
dc.language.isoen
dc.publisherCELL PRESS
dc.relation.ispartofSTRUCTURE
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCell Biology
dc.subjectCENTRAL STALK
dc.subjectCRYSTAL-STRUCTURE
dc.subjectMEMBRANE TOPOLOGY
dc.subjectNA+-ATPASE
dc.subjectROTOR RING
dc.subjectSUBUNIT-C
dc.subjectSYNTHASE
dc.subjectTRANSMEMBRANE SEGMENTS
dc.subjectV-ATPASE
dc.subjectYEAST
dc.titleStructure of the Vacuolar H+-ATPase Rotary Motor Reveals New Mechanistic Insights
dc.typejournal article
dc.identifier.doi10.1016/j.str.2014.12.016
dc.identifier.isiISI:000350886100006
dc.description.volume23
dc.description.issue3
dc.description.startpage461
dc.description.endpage471
dc.contributor.orcid0000-0001-6869-4414
dc.contributor.orcid0000-0002-2973-2630
dc.contributor.orcid0000-0002-7826-7472
dc.contributor.researcheridAAI-9529-2020
dc.identifier.eissn18784186
dc.publisher.place50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA
dcterms.isPartOf.abbreviationStructure
dcterms.oaStatusGreen Published, hybrid
crisitem.author.deptUniversität Osnabrück-
crisitem.author.deptUniversität Osnabrück-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptUniversität Osnabrück-
crisitem.author.deptUniversität Osnabrück-
crisitem.author.deptidfb05-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-2973-2630-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidRaSh001-
crisitem.author.netidPhCl001-
crisitem.author.netidHuMa001-
crisitem.author.netidTiFe418-
crisitem.author.netidWiHe990-
crisitem.author.netidTrJo001-
crisitem.author.netidHaMi001-
crisitem.author.netidMuSt001-
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