Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins

DC FieldValueLanguage
dc.contributor.authorHill, K
dc.contributor.authorModel, K
dc.contributor.authorRyan, MT
dc.contributor.authorDietmeier, K
dc.contributor.authorMartin, F
dc.contributor.authorWagner, R
dc.contributor.authorPfanner, N
dc.date.accessioned2021-12-23T16:07:22Z-
dc.date.available2021-12-23T16:07:22Z-
dc.date.issued1998
dc.identifier.issn00280836
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7846-
dc.description.abstractThe mitochondrial outer membrane contains machinery for the import of preproteins encoded by nuclear genes(1-3). Eight different Tom (translocase of outer membrane) proteins have been identified that function as receptors and/or are related to a hypothetical general import pore. Many mitochondrial membrane channel activities have been described(4-7), including one related to Tim23 of the inner-membrane protein-import system(5); however, the pore-forming subunit(s) of the Tom machinery have not been identified until now. Here we describe the expression and functional reconstitution of Tom40, an integral membrane protein with mainly beta-sheet structure. Tom40 forms a cation-selective high-conductance channel that specifically binds to and transports mitochondrial-targeting sequences added to the cis side of the membrane. We conclude that Tom40 is the pore-forming subunit of the mitochondrial general import pore and that it constitutes a hydrophilic, similar to 22 Angstrom wide channel for the import of preproteins.
dc.language.isoen
dc.publisherMACMILLAN MAGAZINES LTD
dc.relation.ispartofNATURE
dc.subjectCELL VIABILITY
dc.subjectCONTACT SITES
dc.subjectIDENTIFICATION
dc.subjectMACHINERY
dc.subjectMultidisciplinary Sciences
dc.subjectOUTER-MEMBRANE PROTEIN
dc.subjectPRECURSOR PROTEINS
dc.subjectPRESEQUENCE
dc.subjectRECEPTOR COMPLEX
dc.subjectRECOGNITION
dc.subjectScience & Technology - Other Topics
dc.subjectTRANSLOCATION
dc.titleTom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins
dc.typejournal article
dc.identifier.doi10.1038/26780
dc.identifier.isiISI:000076212200060
dc.description.volume395
dc.description.issue6701
dc.description.startpage516
dc.description.endpage521
dc.contributor.orcid0000-0003-2586-8829
dc.contributor.orcid0000-0003-2586-8829
dc.contributor.researcheridC-6673-2011
dc.contributor.researcheridX-2152-2019
dc.contributor.researcheridAAV-7878-2021
dc.publisher.placePORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW
dcterms.isPartOf.abbreviationNature
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWaRi703-
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