Biofunctionalization of carbon nanotubes for reversible site-specific protein immobilization
DC Element | Wert | Sprache |
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dc.contributor.author | Jorde, Lara | |
dc.contributor.author | Li, Zehao | |
dc.contributor.author | Poeppelwerth, Adrian | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | You, Changjiang | |
dc.contributor.author | Meyer, Carola | |
dc.date.accessioned | 2021-12-23T16:07:24Z | - |
dc.date.available | 2021-12-23T16:07:24Z | - |
dc.date.issued | 2021 | |
dc.identifier.issn | 00218979 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/7865 | - |
dc.description.abstract | Carbon nanotubes (CNTs) emerge as biosensors due to their extraordinary electrical transport properties. Such applications depend on interfacing proteins with CNTs in an oriented manner while maintaining their structural and functional integrity, opening versatile opportunities for one-dimensional alignment and high-sensitivity sensing of protein interactions and conformational dynamics. Here, we devised a novel surface architecture for reversible protein immobilization on CNTs via a short peptide tag for fabrication of reusable biosensors for multiple protein analysis. To this end, we conjugated pyrene-poly(ethylene glycol) with tris-nitrilotriacetic acid (Py-PEG-tris-NTA) for site-specific immobilization of oligohistidine(His)-tagged proteins. We demonstrate spontaneous self-assembly of Py-PEG-tris-NTA on CNTs and graphene, as well as specific capturing of His-tagged green fluorescent protein after loading the NTA chelators with Ni(II) ions. The protein binding capacity of tris-NTA-functionalized CNTs could be restored by an imidazole wash, allowing for repeated immobilization cycles. Reflectance interference spectroscopy and fluorescence lifetime analysis confirmed a specific and reversible binding of proteins on CNT-coated silica substrates as well as close interfacing to the CNT surface. | |
dc.description.sponsorship | DFG microscopy facility iBiOs [PI 405/14-1]; Niedersachsisches Vorab [Akz. 11-76251-14-3/15(ZN3141)]; NSFCNational Natural Science Foundation of China (NSFC) [31870976]; Osnabruck University; The authors thank Hella Kenneweg and Annette Budke-Gieseking for expert technical assistance on protein preparation. Fluorescence microscopy was performed with technical support from the DFG microscopy facility iBiOs (No. PI 405/14-1). C.M. acknowledges funding by Niedersachsisches Vorab [No. Akz. 11-76251-14-3/15(ZN3141)]. Z.L. acknowledges the support from NSFC (No. 31870976). This project was supported by intramural funding from the Osnabruck University (Profile line ``Integrated Science,'' C.Y., J.P., and C.M.). | |
dc.language.iso | en | |
dc.publisher | AMER INST PHYSICS | |
dc.relation.ispartof | JOURNAL OF APPLIED PHYSICS | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.title | Biofunctionalization of carbon nanotubes for reversible site-specific protein immobilization | |
dc.type | journal article | |
dc.identifier.doi | 10.1063/5.0035871 | |
dc.identifier.isi | ISI:000630423600002 | |
dc.description.volume | 129 | |
dc.description.issue | 9 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.orcid | 0000-0003-0851-2767 | |
dc.contributor.orcid | 0000-0002-6813-6313 | |
dc.contributor.researcherid | I-1723-2012 | |
dc.identifier.eissn | 10897550 | |
dc.publisher.place | 1305 WALT WHITMAN RD, STE 300, MELVILLE, NY 11747-4501 USA | |
dcterms.isPartOf.abbreviation | J. Appl. Phys. | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.orcid | 0000-0003-0851-2767 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | PiJa938 | - |
crisitem.author.netid | YoCh745 | - |
crisitem.author.netid | MeCa197 | - |
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geprüft am 15.05.2024