Native Laser Lithography of His-Tagged Proteins by Uncaging of Multivalent Chelators
DC Element | Wert | Sprache |
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dc.contributor.author | Bhagawati, Maniraj | |
dc.contributor.author | Lata, Suman | |
dc.contributor.author | Tampe, Robert | |
dc.contributor.author | Piehler, Jacob | |
dc.date.accessioned | 2021-12-23T16:07:30Z | - |
dc.date.available | 2021-12-23T16:07:30Z | - |
dc.date.issued | 2010 | |
dc.identifier.issn | 00027863 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/7906 | - |
dc.description.abstract | We report a generic approach for targeting proteins into micropatterns by in situ laser Lithography. To this end, we have designed a photocleavable oligohistidine peptide for caging tris(nitrilo triacetic acid) (tris-NTA) groups on surfaces by multivalent interactions. Local photofragmentation of the peptide by UV illumination through a photomask or by a confocal laser beam uncages tris-NTA, thus generating free binding sites for rapid, site-specific capturing of His-tagged proteins into micropatterns. Iterative writing of proteins by laser lithography enabled for assembly of multiplexed functional protein microstructures on surfaces. Thus, versatile, user-defined protein micropatterns can be assembled under physiological conditions with a standard confocal laser-scanning microscope. | |
dc.description.sponsorship | BMBFFederal Ministry of Education & Research (BMBF) [0312034, 0312031]; DFGGerman Research Foundation (DFG)European Commission [PI 405/4, PI 405/3]; Photomasks were obtained from NB-Technologies, Bremen. This project was supported by the BMBF (0312034, 0312031) and by the DFG (PI 405/4). J.P was a Heisenberg-Professor funded by the DFG, 2007-2008 (PI 405/3). | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | |
dc.subject | BIOMOLECULES | |
dc.subject | BIOTIN | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | FUNCTIONAL IMMOBILIZATION | |
dc.subject | MOTOR PROTEINS | |
dc.subject | STREPTAVIDIN | |
dc.subject | SURFACES | |
dc.title | Native Laser Lithography of His-Tagged Proteins by Uncaging of Multivalent Chelators | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/ja1000714 | |
dc.identifier.isi | ISI:000277158500005 | |
dc.description.volume | 132 | |
dc.description.issue | 17 | |
dc.description.startpage | 5932+ | |
dc.contributor.orcid | 0000-0002-0403-2160 | |
dc.contributor.researcherid | H-2953-2015 | |
dc.identifier.eissn | 15205126 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | J. Am. Chem. Soc. | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 04.05.2024