Origin of a chloroplast protein importer

DC FieldValueLanguage
dc.contributor.authorBolter, B
dc.contributor.authorSoll, J
dc.contributor.authorSchulz, A
dc.contributor.authorHinnah, S
dc.contributor.authorWagner, R
dc.date.accessioned2021-12-23T16:07:34Z-
dc.date.available2021-12-23T16:07:34Z-
dc.date.issued1998
dc.identifier.issn00278424
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/7938-
dc.description.abstractDuring evolution, chloroplasts have relinquished the majority of their genes to the nucleus. The products of transferred genes are imported into the organelle with the help of an import machinery that is distributed across the inner and outer plastid membranes. The evolutionary origin of this machinery is puzzling because, in the putative predecessors, the cyanobacteria, the outer two membranes, the plasma membrane, and the lipopolysaccharide layer lack a functionally similar protein import system. A 75-kDa protein-conducting channel in the outer envelope of pea chloroplasts, Toc75, shares approximate to 22% amino acid identity to a similarly sized protein, designated SynToc75, encoded in the Synechocystis PCC6803 genome, Here we show that SynToc75 is located in the outer membrane (lipopolysaccharide layer) of Synechocystis PCC6803 and that SynToc75 forms a voltage-gated, high conductance channel with a high affinity for polyamines and peptides in reconstituted liposomes, These findings suggest that a component of the chloroplast protein import system, Toc75, was recruited from a preexisting channel-forming protein of the cyanobacterial outer membrane. Furthermore, the presence of a protein in the chloroplastic outer envelope homologous to a cyanobacterial protein provides support for the prokaryotic nature of this chloroplastic membrane.
dc.language.isoen
dc.publisherNATL ACAD SCIENCES
dc.relation.ispartofPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
dc.subjectAPPARATUS
dc.subjectCOMPONENTS
dc.subjectENDOPLASMIC-RETICULUM
dc.subjectENVELOPE MEMBRANE
dc.subjectGENE
dc.subjectINTERACT
dc.subjectMACHINERY
dc.subjectMultidisciplinary Sciences
dc.subjectOUTER-MEMBRANE
dc.subjectPRECURSOR PROTEINS
dc.subjectScience & Technology - Other Topics
dc.subjectTRANSLOCATION
dc.titleOrigin of a chloroplast protein importer
dc.typejournal article
dc.identifier.doi10.1073/pnas.95.26.15831
dc.identifier.isiISI:000077697200123
dc.description.volume95
dc.description.issue26
dc.description.startpage15831
dc.description.endpage15836
dc.contributor.orcid0000-0001-6796-3598
dc.contributor.researcheridA-3357-2013
dc.publisher.place2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA
dcterms.isPartOf.abbreviationProc. Natl. Acad. Sci. U. S. A.
dcterms.oaStatusGreen Published, Bronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWaRi703-
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