Chemical analysis of isolated cell walls of Gram-positive bacteria and determination of the cell wall to cell mass ratio

Autor(en): vanderWal, A
Norde, W
Bendinger, B
Zehnder, AJB
Lyklema, J
Stichwörter: ADHESION; Bacillus brevis; Biochemical Research Methods; Biochemistry & Molecular Biology; cell wall composition; cell wall isolation; cell wall mass; CORYNEFORM BACTERIA; DEGRADATION; Microbiology; MYCOLIC ACIDS; PEPTIDOGLYCAN; SP-NOV; SURFACE
Erscheinungsdatum: 1997
Herausgeber: ELSEVIER SCIENCE BV
Journal: JOURNAL OF MICROBIOLOGICAL METHODS
Volumen: 28
Ausgabe: 2
Startseite: 147
Seitenende: 157
Zusammenfassung: 
Cell walls of five Gram-positive bacterial strains, including four coryneforms and a Bacillus brevis strain were isolated and subsequently chemically analysed. The wall contribution to the total cell mass is calculated from a comparison of D-Lactate concentrations in hydrolysates of whole cells and isolated walls. D-lactate concentrations are measured enzymatically after purification of the samples with active carbon. The optimum condition for the quantitative elimination of D-lactate from the peptidoglycan appears to be at 120 degrees C and 4 M HCl. The wall fraction for the cells of the coryneform bacteria are in the range of 26-32% and is about 75% for the B. brevis cells. The peptidoglycan fraction in the walls of the coryneform bacteria was found to be rather high and constitutes about 23 to 31% of the cell wall dry weight. The protein content of the cell wall dry weight is somewhat lower and in the range of 7 to 14%. The peptidoglycan in the wall of the B. brevis strain forms a thin layer and contributes only about 5% to the cell wall dry weight. The high amount of proteins in the B. brevis cell wall (>56%) can be attributed to a so called S(urface)-layer. (C) 1997 Elsevier Science B.V.
ISSN: 01677012
DOI: 10.1016/S0167-7012(97)00981-0

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