Cold shock stress-induced proteins in Bacillus subtilis

DC FieldValueLanguage
dc.contributor.authorGraumann, P
dc.contributor.authorSchroder, K
dc.contributor.authorSchmid, R
dc.contributor.authorMarahiel, MA
dc.date.accessioned2021-12-23T16:07:44Z-
dc.date.available2021-12-23T16:07:44Z-
dc.date.issued1996
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8028-
dc.description.abstractBacteria respond to a decrease in temperature with the induction of proteins that are classified as cold-induced proteins (CIPs). Using two-dimensional gel electrophoresis, we analyzed the cold shock response in Bacillus subtilis. After a shift from 37 to 15 degrees C, the synthesis of a majority of proteins was repressed; in contrast, 37 proteins were synthesized at rates higher than preshift rates. One hour after cold shock, the induction of CIPs decreased, and after 2 h, general protein synthesis resumed. The identified main CIPs were excised from two-dimensional gels and were subjected to microsequencing. Three small acidic proteins that showed the highest relative induction after cold shock were highly homologous and belonged to a protein family of which one member, the major cold shock protein, CspB, has previously been characterized. Two-dimensional gel analyses of a cspB null mutant revealed that CspB affects the level of induction of several CIPs. Other identified CIPs function at various levels of cellular physiology, such as chemotaxis (CheY), sugar uptake (Hpr), translation (ribosomal proteins S6 and L7/L12), protein folding (PPiB), and general metabolism (CysK, IlvC, Gap, and triosephosphate isomerase).
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectBINDING
dc.subjectCRYSTAL-STRUCTURE
dc.subjectCSPA
dc.subjectESCHERICHIA-COLI
dc.subjectGENE
dc.subjectHEAT
dc.subjectIDENTIFICATION
dc.subjectLOW-TEMPERATURE
dc.subjectMicrobiology
dc.subjectSEQUENCE
dc.subjectTRANSCRIPTIONAL ANALYSIS
dc.titleCold shock stress-induced proteins in Bacillus subtilis
dc.typejournal article
dc.identifier.doi10.1128/jb.178.15.4611-4619.1996
dc.identifier.isiISI:A1996UZ04700033
dc.description.volume178
dc.description.issue15
dc.description.startpage4611
dc.description.endpage4619
dc.contributor.orcid0000-0002-8033-5171
dc.identifier.eissn10985530
dc.publisher.place1752 N ST NW, WASHINGTON, DC 20036-2904 USA
dcterms.isPartOf.abbreviationJ. Bacteriol.
dcterms.oaStatusBronze, Green Published
Show simple item record

Google ScholarTM

Check

Altmetric