Two novel calcium-binding proteins from cytoplasmic granules of the protozoan parasite Entamoeba histolytica

Autor(en): Nickel, R
Jacobs, T 
Urban, B
Scholze, H
Bruhn, H
Leippe, M
Stichwörter: AMOEBAPORES; Biochemistry & Molecular Biology; Biophysics; calcium-binding protein; Cell Biology; CLONING; cytoplasmic granule; EF-hand; Entamoeba histolytica; GENE; PEPTIDES; SEQUENCE-ANALYSIS
Erscheinungsdatum: 2000
Herausgeber: ELSEVIER SCIENCE BV
Journal: FEBS LETTERS
Volumen: 486
Ausgabe: 2
Startseite: 112
Seitenende: 116
Zusammenfassung: 
We report on the molecular characterisation of two novel granule proteins of the protozoon and human pathogen Entamoeba histolytica. The proteins, which were named grainin 1 and 2, show a considerable structural similarity to calcium-binding proteins, particularly within EF-hand motifs. Each grainin possesses three of these putative calcium-binding sites. Based on careful inspection of known structures of protein families containing EF-hands, a domain of grainin 1 covering two EF-band motifs was modeled by homology. Calcium-binding activity of grainins was demonstrated by two independent methods. These granule proteins may be implicated in functions vital for the primitive phagocyte and destructive parasite such as control of endocytotic pathways and granule discharge. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
ISSN: 00145793
DOI: 10.1016/S0014-5793(00)02245-6

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