The phosphotransferase system of Streptomyces coelicolor IIA(Crr) exhibits properties that resemble transport and inducer exclusion function of enzyme IIA(Glucose) of Escherichia coli

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dc.contributor.authorKamionka, A
dc.contributor.authorParche, S
dc.contributor.authorNothaft, H
dc.contributor.authorSiepelmeyer, J
dc.contributor.authorJahreis, K
dc.contributor.authorTitgemeyer, F
dc.date.accessioned2021-12-23T16:07:58Z-
dc.date.available2021-12-23T16:07:58Z-
dc.date.issued2002
dc.identifier.issn00142956
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8156-
dc.description.abstractWe have investigated the err gene of Streptomyces coelicolor that encodes a homologue of enzyme IIA(Glucose) of Escherichia coli, which, as a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) plays a key role in carbon regulation by triggering glucose transport, carbon catabolite repression, and inducer exclusion. As in E. coli, the err gene of S. coelicolor is genetically associated with the ptsI gene that encodes the general phosphotransferase enzyme I. The gene product IIA(Crr) was overproduced, purified, and polyclonal antibodies were obtained. Western blot analysis revealed that IIA(Crr) is expressed in vivo. The functionality of IIA(Crr) was demonstrated by phosphoenolpyruvate-dependent phosphorylation via enzyme I and the histidine-containing phosphoryl carrier protein HPr. Phosphorylation was abolished when His72, which corresponds to the catalytic histidine of E. coli IIA(Glucose), was mutated. The capacity of IIA(Crr) to operate in sugar transport was shown by complementation of the E. coli glucose-PTS. The striking functional resemblance between IIA(Crr) and IIA(Glucose) was further demonstrated by its ability to confer inducer exclusion of maltose to E. coli. A specific interaction of IIA(Crr) with the maltose permease subunit MalK from Salmonella typhimurium was uncovered by surface plasmon resonance. These data suggest that this IIA(Gluose)-like protein may be involved in carbon metabolism in S. coelicolor.
dc.language.isoen
dc.publisherBLACKWELL PUBLISHING LTD
dc.relation.ispartofEUROPEAN JOURNAL OF BIOCHEMISTRY
dc.subjectANTIBIOTIC PRODUCTION
dc.subjectBACILLUS-SUBTILIS
dc.subjectBIOCHEMICAL-ANALYSIS
dc.subjectBiochemistry & Molecular Biology
dc.subjectCARBON CATABOLITE REPRESSION
dc.subjectGENE ENCODES
dc.subjectGLUCOSE KINASE
dc.subjectinducer exclusion
dc.subjectPHOSPHOCARRIER PROTEIN
dc.subjectprotein phosphorylation
dc.subjectprotein-protein interaction
dc.subjectPTS VIEW
dc.subjectREGULATORY ROLE
dc.subjectSITE-DIRECTED MUTAGENESIS
dc.subjectStreptomyces
dc.subjectsurface plasmon resonance
dc.titleThe phosphotransferase system of Streptomyces coelicolor IIA(Crr) exhibits properties that resemble transport and inducer exclusion function of enzyme IIA(Glucose) of Escherichia coli
dc.typejournal article
dc.identifier.doi10.1046/j.1432-1033.2002.02864.x
dc.identifier.isiISI:000175410900019
dc.description.volume269
dc.description.issue8
dc.description.startpage2143
dc.description.endpage2150
dc.publisher.place9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND
dcterms.isPartOf.abbreviationEur. J. Biochem.
dcterms.oaStatusBronze
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